ArticleMolecular biology of the cell1999
Birefringence imaging directly reveals architectural dynamics of filamentous actin in living growth cones.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
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- Initiation and disassembly of filopodia tip complexes containing VASP and lamellipodin.Molecular biology of the cell · 2020Article
- Birefringence Changes of Dendrites in Mouse Hippocampal Slices Revealed with Polarizing Microscopy.Biophysical journal · 2020Article
- Forces to Drive Neuronal Migration Steps.Frontiers in cell and developmental biology · 2020Review
- Microscale Interrogation of 3D Tissue Mechanics.Frontiers in bioengineering and biotechnology · 2019Review
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- Single-Shot Optical Anisotropy Imaging with Quantitative Polarization Interference Microscopy.Laser & photonics reviews · 2018Article
- Shootin1-cortactin interaction mediates signal-force transduction for axon outgrowth.The Journal of cell biology · 2015Article
- Compound antimalarial ethosomal cataplasm: preparation, evaluation, and mechanism of penetration enhancement.International journal of nanomedicine · 2015Article
- Trimodal color-fluorescence-polarization endoscopy aided by a tumor selective molecular probe accurately detects flat lesions in colitis-associated cancer.Journal of biomedical optics · 2014Article
- Ionizing radiation effects on the secretory-stage ameloblasts and enamel organic extracellular matrix.Radiation and environmental biophysics · 2014Article
- Broadening the spectrum of actin-based protrusive activity mediated by Arp2/3 complex-facilitated polymerization: motility of cytoplasmic ridges and tubular projections.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Organization of FtsZ filaments in the bacterial division ring measured from polarized fluorescence microscopy.Biophysical journal · 2013Article
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4 authors.
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Abstract
We have investigated the dynamic behavior of cytoskeletal fine structure in the lamellipodium of nerve growth cones using a new type of polarized light microscope (the Pol-Scope). Pol-Scope images display with exquisite resolution and definition birefringent fine structures, such as filaments and membranes, without having to treat the cell with exogenous dyes or fluorescent labels. Furthermore, the measured birefringence of protein fibers in the thin lamellipodial region can be interpreted in terms of the number of filaments in the bundles. We confirmed that birefringent fibers are actin-based using conventional fluorescence-labeling methods. By recording movies of time-lapsed Pol-Scope images, we analyzed the creation and dynamic composition of radial fibers, filopodia, and intrapodia in advancing growth cones. The strictly quantitative information available in time-lapsed Pol-Scope images confirms previously deduced behavior and provides new insight into the architectural dynamics of filamentous actin.
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