ArticleMolecular biology of the cell1999
Sequences within the cytoplasmic domain of gp180/carboxypeptidase D mediate localization to the trans-Golgi network.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Systematic Quantification of Protein O-GlcNAcylation Reveals Common and Cell-Type-Specific Responses to N-Glycosylation Inhibition in Human Cells.Analytical chemistry · 2026Article
- Cargo selective vesicle tethering: The structural basis for binding of specific cargo proteins by the Golgi tether component TBC1D23.Science advances · 2024Article
- Article
- Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production.Molecular biology of the cell · 2020Article
- Contribution of the clathrin adaptor AP-1 subunit µ1 to acidic cluster protein sorting.The Journal of cell biology · 2017Article
- Substrate specificity of human metallocarboxypeptidase D: Comparison of the two active carboxypeptidase domains.PloS one · 2017Article
- From DCPD to NTCP: the long journey towards identifying a functional hepatitis B virus receptor.Clinical and molecular hepatology · 2015Review
- Novel carboxypeptidase A6 (CPA6) mutations identified in patients with juvenile myoclonic and generalized epilepsy.PloS one · 2015Article
- AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.Traffic (Copenhagen, Denmark) · 2014Article
- Intracellular α(2C)-adrenoceptors: storage depot, stunted development or signaling domain?Biochimica et biophysica acta · 2011Review
- Initiation of duck hepatitis B virus infection requires cleavage by a furin-like protease.Journal of virology · 2010Article
- Peptidomics of Cpe(fat/fat) mouse brain regions: implications for neuropeptide processing.Journal of neurochemistry · 2008Article
- HIV-1 Nef-induced down-regulation of MHC class I requires AP-1 and clathrin but not PACS-1 and is impeded by AP-2.Molecular biology of the cell · 2007Article
- Mapping the landscape of the lymphocytic choriomeningitis virus stable signal peptide reveals novel functional domains.Journal of virology · 2007Article
- Viral and cellular determinants involved in hepadnaviral entry.World journal of gastroenterology · 2007Review
- Endocytosis plays a critical role in proteolytic processing of the Hendra virus fusion protein.Journal of virology · 2005Article
- Characterization of a novel, cytokine-inducible carboxypeptidase D isoform in haematopoietic tumour cells.The Biochemical journal · 2005Article
- Redistribution of cellular and herpes simplex virus proteins from the trans-golgi network to cell junctions without enveloped capsids.Journal of virology · 2004Article
- Membrane-bound carboxypeptidase E facilitates the entry of eosinophil cationic protein into neuroendocrine cells.The Biochemical journal · 2004Article
- Interaction of phospholipase D1 with a casein-kinase-2-like serine kinase.The Biochemical journal · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gp180, a duck protein that was proposed to be a cell surface receptor for duck hepatitis B virus, is the homolog of metallocarboxypeptidase D, a mammalian protein thought to function in the trans-Golgi network (TGN) in the processing of proteins that transit the secretory pathway. Both gp180 and mammalian metallocarboxypeptidase D are type I integral membrane proteins that contain a 58-residue cytosolic C-terminal tail that is highly conserved between duck and rat. To investigate the regions of the gp180 tail involved with TGN retention and intracellular trafficking, gp180 and various deletion and point mutations were expressed in the AtT-20 mouse pituitary corticotroph cell line. Full length gp180 is enriched in the TGN and also cycles to the cell surface. Truncation of the C-terminal 56 residues of the cytosolic tail eliminates the enrichment in the TGN and the retrieval from the cell surface. Truncation of 12-43 residues of the tail reduced retention in the TGN and greatly accelerated the turnover of the protein. In contrast, deletion of the C-terminal 45 residues, which truncates a potential YxxL-like sequence (FxxL), reduced the protein turnover and caused accumulation of the protein on the cell surface. A point mutation of the FxxL sequence to AxxL slowed internalization, showing that this element is important for retrieval from the cell surface. Mutation of a pair of casein kinase II sites within an acidic cluster showed that they are also important for trafficking. The present study demonstrates that multiple sequence elements within the cytoplasmic tail of gp180 participate in TGN localization.
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