ArticleThe EMBO journal1999
Production of aberrant promoter transcripts contributes to methylation and silencing of unlinked homologous promoters in trans.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 227 citations in OpenAlex.
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- Noncoding RNAs as tools for advancing translational biology in plants.The Plant cell · 2025Review
- A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants.Transgenic research · 2023Article
- Long distance signalling and epigenetic changes in crop grafting.Frontiers in plant science · 2023Review
- A deletion/duplication in the Ligon lintless-2 locus induces siRNAs that inhibit cotton fiber cell elongation.Plant physiology · 2022Article
- A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.Plant physiology · 2022Article
- Will epigenetics be a key player in crop breeding?Frontiers in plant science · 2022Review
- Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene.International journal of molecular sciences · 2021Article
- RNA Interference (RNAi) Screening in Cultured Drosophila Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- DNA demethylase ROS1 prevents inheritable DREB1A/CBF3 repression by transgene-induced promoter methylation in the Arabidopsis ice1-1 mutant.Plant molecular biology · 2020Article
- Epigenetic Modifications: An Unexplored Facet of Exogenous RNA Application in Plants.Plants (Basel, Switzerland) · 2020Article
- Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants.Plant physiology · 2020Article
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- Transgenic creeping bentgrass plants expressing a Picea wilsonii dehydrin gene (PicW) demonstrate improved freezing tolerance.Molecular biology reports · 2018Article
- ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals.Molecular cell · 2018Article
- Expression characterization of the herbicide tolerance gene Aryloxyalkanoate Dioxygenase (aad-1) controlled by seven combinations of regulatory elements.BMC plant biology · 2018Article
- Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption.Frontiers in plant science · 2018Article
- Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding.Genome biology · 2017Article
- Novel strong promoter of antimicrobial peptides gene pro-SmAMP2 from chickweed (Stellaria media).BMC biotechnology · 2016Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous work has suggested that de novo methylation of plant nuclear genes can be triggered by an RNA-DNA interaction. To test whether transcription of a promoter would induce de novo methylation and silencing of unlinked genes driven by the same promoter, a chimeric 'gene' consisting of a nopaline synthase promoter (NOSpro) positioned downstream of the cauliflower mosaic virus 35S promoter (35Spro) and flanked at the 3' end by a NOS terminator (NOSter) was constructed and introduced into the genome of a plant that normally expresses an unmethylated NOSpro-neomycinphosphotransferase (nptII) gene. Transformants were tested for kanamycin resistance and NOSpro RNA synthesis. Most produced a full-length polyadenylated NOSpro RNA, which did not induce silencing or methylation at the NOSpro-nptII target gene. One, however, contained truncated non-polyadenylated NOSpro RNA; in this plant, the NOSpro-nptII gene became silenced and methylated in the NOSpro region. Molecular analysis of the NOSpro silencing locus revealed two incomplete copies of the 35Spro-NOSpro gene arranged as an inverted repeat with NOSpro sequences at the center. Reducing NOSpro transcription by crossing a 35Spro-silencing locus partially reactivated nptII gene expression and decreased NOSpro methylation at the target locus, thus implicating aberrant NOSpro RNA in this trans-silencing phenomenon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.