ArticleBiophysical journal1999
Bacterial S-layer protein coupling to lipids: x-ray reflectivity and grazing incidence diffraction studies.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- New Insights into the Molecular Structure of Tear Film Lipids Revealed by Surface X-ray Scattering.The journal of physical chemistry letters · 2024Article
- X-ray reflectivity study of the heat shock protein Hsp70 interaction with an artificial cell membrane model.Scientific reports · 2023Article
- Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges.International journal of nanomedicine · 2019Review
- Sensitivity of peripheral membrane proteins to the membrane context: A case study of phosphatidylserine and the TIM proteins.Biochimica et biophysica acta. Biomembranes · 2018Review
- S-Layer Protein-Based Biosensors.Biosensors · 2018Article
- Zooming in on disordered systems: neutron reflection studies of proteins associated with fluid membranes.Biochimica et biophysica acta · 2014Review
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules.Journal of the Royal Society, Interface · 2014Review
- Small-angle X-ray scattering for imaging of surface layers on intact bacteria in the native environment.Journal of bacteriology · 2013Article
- S-layer protein self-assembly.International journal of molecular sciences · 2013Article
- Measuring Ca2+-induced structural changes in lipid monolayers: implications for synaptic vesicle exocytosis.Biophysical journal · 2012Article
- Biosensing for the environment and defence: aqueous uranyl detection using bacterial surface layer proteins.Sensors (Basel, Switzerland) · 2010Article
- Composite S-layer lipid structures.Journal of structural biology · 2009Review
- Part II: diffraction from two-dimensional cholera toxin crystals bound to their receptors in a lipid monolayer.Biophysical journal · 2008Article
- S-layer stabilized lipid membranes (Review).Biointerphases · 2008Article
- X-ray reflectivity studies of cPLA2{alpha}-C2 domains adsorbed onto Langmuir monolayers of SOPC.Biophysical journal · 2005Article
- Cholera toxin assault on lipid monolayers containing ganglioside GM1.Biophysical journal · 2004Article
- The effect of S-layer protein adsorption and crystallization on the collective motion of a planar lipid bilayer studied by dynamic light scattering.Biophysical journal · 1999Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The coupling of bacterial surface (S)-layer proteins to lipid membranes is studied in molecular detail for proteins from Bacillus sphaericus CCM2177 and B. coagulans E38-66 recrystallized at dipalmitoylphosphatidylethanolamine (DPPE) monolayers on aqueous buffer. A comparison of the monolayer structure before and after protein recrystallization shows minimal reorganization of the lipid chains. By contrast, the lipid headgroups show major rearrangements. For the B. sphaericus CCM2177 protein underneath DPPE monolayers, x-ray reflectivity data suggest that amino acid side chains intercalate the lipid headgroups at least to the phosphate moieties, and probably further beyond. The number of electrons in the headgroup region increases by more than four per lipid. Analysis of the changes of the deduced electron density profiles in terms of a molecular interpretation shows that the phosphatidylethanolamine headgroups must reorient toward the surface normal to accommodate such changes. In terms of the protein structure (which is as yet unknown in three dimensions), the electron density profile reveals a thickness lz approximately 90 A of the recrystallized S-layer and shows water-filled cavities near its center. The protein volume fraction reaches maxima of >60% in two horizontal sections of the S-layer, close to the lipid monolayer and close to the free subphase. In between it drops to approximately 20%. Four S-layer protein monomers are located within the unit cell of a square lattice with a spacing of approximately 131 A.
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