ArticleBiophysical journal1999
Gbetagamma binding increases the open time of IKACh: kinetic evidence for multiple Gbetagamma binding sites.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Neuronal G protein-gated KAmerican journal of physiology. Cell physiology · 2022Review
- Structural mechanism of TRPM7 channel regulation by intracellular magnesium.Cellular and molecular life sciences : CMLS · 2022Article
- Dimethylethanolamine Decreases Epileptiform Activity in Acute Human Hippocampal SlicesFrontiers in molecular neuroscience · 2019Article
- Downregulation of BK channel function and protein expression in coronary arteriolar smooth muscle cells of type 2 diabetic patients.Cardiovascular research · 2019Article
- A Quantitative Model of the GIRK1/2 Channel Reveals That Its Basal and Evoked Activities Are Controlled by Unequal Stoichiometry of Gα and Gβγ.PLoS computational biology · 2015Article
- Interactions of cations with the cytoplasmic pores of inward rectifier K(+) channels in the closed state.The Journal of biological chemistry · 2011Article
- Emerging roles for G protein-gated inwardly rectifying potassium (GIRK) channels in health and disease.Nature reviews. Neuroscience · 2010Review
- G alpha(i) and G betagamma jointly regulate the conformations of a G betagamma effector, the neuronal G protein-activated K+ channel (GIRK).The Journal of biological chemistry · 2010Article
- Amplitude histogram-based method of analysis of patch clamp recordings that involve extreme changes in channel activity levels.Journal of molecular neuroscience : MN · 2009Article
- Synthesis, trafficking, and localization of muscarinic acetylcholine receptors.Pharmacology & therapeutics · 2008Review
- Base of pore loop is important for rectification, activation, permeation, and block of Kir3.1/Kir3.4.Biophysical journal · 2006Article
- Modulation of midbrain dopamine neurotransmission by serotonin, a versatile interaction between neurotransmitters and significance for antipsychotic drug action.Current neuropharmacology · 2006Article
- Spinal G-protein-gated potassium channels contribute in a dose-dependent manner to the analgesic effect of mu- and delta- but not kappa-opioids.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Properties and modulation of the G protein-coupled K+ channel in rat cerebellar granule neurons: ATP versus phosphatidylinositol 4,5-bisphosphate.The Journal of physiology · 2003Article
- The pore helix is involved in stabilizing the open state of inwardly rectifying K+ channels.Biophysical journal · 2003Article
- Single channel analysis of the regulation of GIRK1/GIRK4 channels by protein phosphorylation.Biophysical journal · 2003Article
- Gating properties of GIRK channels activated by Galpha(o)- and Galpha(i)-coupled muscarinic m2 receptors in Xenopus oocytes: the role of receptor precoupling in RGS modulation.The Journal of physiology · 2002Article
- Somatostatin inhibits thalamic network oscillations in vitro: actions on the GABAergic neurons of the reticular nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Phasic and tonic attenuation of EPSPs by inward rectifier K+ channels in rat hippocampal pyramidal cells.The Journal of physiology · 2002Article
- Slow modal gating of single G protein-activated K+ channels expressed in Xenopus oocytes.The Journal of physiology · 2000Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IKACh is an inwardly rectifying potassium channel that plays an important role in the regulation of mammalian heart rate. IKACh is activated by direct interaction with Gbetagamma subunits of pertussis toxin-sensitive heterotrimeric G-proteins. The stoichiometry of the Gbetagamma/channel complex is currently unknown, and kinetic analysis of the channel behavior has led to conflicting conclusions. Here, we analyze the kinetics of the native IKACh channel in inside-out cardiomyocyte patches activated directly by Gbetagamma. We conclude that the channel has at least two open states and that binding of Gbetagamma prolongs its mean open time duration. These findings imply the existence of at least two binding sites on the channel complex for Gbetagamma. We also show that the duration of the channel opening is negatively correlated with the duration of subsequent channel closing, which further constrains the possible kinetic models. A simple qualitative model describing the kinetic behavior of IKACh is presented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.