ArticleProceedings of the National Academy of Sciences of the United States of America1999
Emergence of a dual-catalytic RNA with metal-specific cleavage and ligase activities: the spandrels of RNA evolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- RNA ligase ribozymes with a small catalytic core.Scientific reports · 2023Article
- Molecular trade-offs in RNA ligases affected the modular emergence of complex ribozymes at the origin of life.Royal Society open science · 2017Article
- Mapping L1 ligase ribozyme conformational switch.Journal of molecular biology · 2012Article
- Oxytricha as a modern analog of ancient genome evolution.Trends in genetics : TIG · 2012Review
- A small ribozyme with dual-site kinase activity.Nucleic acids research · 2012Article
- Evolution of functional nucleic acids in the presence of nonheritable backbone heterogeneity.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Identification of dynamical hinge points of the L1 ligase molecular switch.RNA (New York, N.Y.) · 2010Article
- Tailoring RNA modular units on a common scaffold: a modular ribozyme with a catalytic unit for beta-nicotinamide mononucleotide-activated RNA ligation.RNA (New York, N.Y.) · 2009Article
- Structure-guided engineering of the regioselectivity of RNA ligase ribozymes.Journal of the American Chemical Society · 2009Article
- The origin of modern terrestrial life.HFSP journal · 2007Article
- Modular evolution and increase of functional complexity in replicating RNA molecules.RNA (New York, N.Y.) · 2007Article
- Characterization of long RNA-cleaving deoxyribozymes with short catalytic cores: the effect of excess sequence elements on the outcome of in vitro selection.Nucleic acids research · 2006Article
- The RNA world on ice: a new scenario for the emergence of RNA information.Journal of molecular evolution · 2005Review
- Diverse Mechanisms of RNA Recombination.Molecular biology · 2005Article
- Ligation activity of fragmented ribozymes in frozen solution: implications for the RNA world.Nucleic acids research · 2004Article
- Evolutionary landscapes for the acquisition of new ligand recognition by RNA aptamers.Journal of molecular evolution · 2003Article
- Optimization and optimality of a short ribozyme ligase that joins non-Watson-Crick base pairings.RNA (New York, N.Y.) · 2001Article
- The effect of cytidine on the structure and function of an RNA ligase ribozyme.RNA (New York, N.Y.) · 2001Article
- Quantitative studies of Mn(2+)-promoted specific and non-specific cleavages of a large RNA: Mn(2+)-GAAA ribozymes and the evolution of small ribozymes.Nucleic acids research · 2000Article
- Testing ancient RNA-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro selection, or directed molecular evolution, allows the isolation and amplification of rare sequences that satisfy a functional-selection criterion. This technique can be used to isolate novel ribozymes (RNA enzymes) from large pools of random sequences. We used in vitro evolution to select a ribozyme that catalyzes a novel template-directed RNA ligation that requires surprisingly few nucleotides for catalytic activity. With the exception of two nucleotides, most of the ribozyme contributes to a template, suggesting that it is a general prebiotic ligase. More surprisingly, the catalytic core built from randomized sequences actually contains a 7-nt manganese-dependent self-cleavage motif originally discovered in the Tetrahymena group I intron. Further experiments revealed that we have selected a dual-catalytic RNA from random sequences: the RNA promotes both cleavage at one site and ligation at another site, suggesting two conformations surrounding at least one divalent metal ion-binding site. Together, these results imply that similar catalytic RNA motifs can arise under fairly simple conditions and that multiple catalytic structures, including bifunctional ligases, can evolve from very small preexisting parts. By breaking apart and joining different RNA strands, such ribozymes could have led to the production of longer and more complex RNA polymers in prebiotic evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.