ArticleProceedings of the National Academy of Sciences of the United States of America1999
Centrosomal control of microtubule dynamics.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed.
- Noncentrosomal Microtubule Assembly and Its Regulation.Cytoskeleton (Hoboken, N.J.) · 2026Review
- Cell division cycle 20 promotes tumor progression and predicts poor clinical outcome in childhood and adult adrenocortical carcinoma.Journal of clinical & translational endocrinology · 2025Article
- Microtubule-Targeting Agents: Disruption of the Cellular Cytoskeleton as a Backbone of Ovarian Cancer Therapy.Advances in experimental medicine and biology · 2024Review
- Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines.International journal of molecular sciences · 2022Article
- Persistent growth of microtubules at low density.Molecular biology of the cell · 2021Article
- Dual Host-Intracellular Parasite Transcriptome of Enucleated Cells HostingInfection and immunity · 2020Article
- The nucleus acts as a ruler tailoring cell responses to spatial constraints.Science (New York, N.Y.) · 2020Article
- Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Loss of Neogenin1 in human colorectal carcinoma cells causes a partial EMT and wound-healing response.Scientific reports · 2019Article
- Non-centrosomal MTs play a crucial role in organization of MT array in interphase fibroblasts.AIMS genetics · 2018Article
- Centrosome defines the rear of cells during mesenchymal migration.Molecular biology of the cell · 2017Article
- Stimulation of microtubule-based transport by nucleation of microtubules on pigment granules.Molecular biology of the cell · 2017Article
- Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration.Cell discovery · 2017Article
- Article
- Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles.PloS one · 2015Article
- Regulation of microtubule minus-end dynamics by CAMSAPs and Patronin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- CAMSAPs add to the growing microtubule minus-end story.Developmental cell · 2014Article
- A non-canonical mode of microtubule organization operates throughout pre-implantation development in mouse.Cell cycle (Georgetown, Tex.) · 2013Article
- Article
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Authors and funding
3 authors.
Funding
Abstract
In many animal cells, minus ends of microtubules (MTs) are thought to be capped by the centrosome whereas plus ends are free and display dynamic instability. We tested the role of the centrosome by examining MT behavior in cytoplasts from which the centrosome was removed. Cells were injected with Cy3-tubulin to fluorescently label MTs and were enucleated by using a centrifugation procedure. Enucleation resulted in a mixture of cytoplasts containing or lacking the centrosome. Fibroblast (CHO-K1) and epithelial (BSC-1) cells were investigated. In fibroblast cytoplasts containing the centrosome, MTs showed dynamic instability indistinguishable from that in intact cells. In contrast, in cytoplasts lacking the centrosome, MTs treadmilled-shortened at the minus end at about 12 micrometers/min while growing at the plus end at the same rate. The change in behavior of the plus end from dynamic instability to persistent growth correlated with an elevated level of free tubulin subunits (78% in centrosome-free cytoplasts vs. 44% in intact cells) generated by minus-end depolymerization. In contrast to fibroblast cells, in centrosome-free cytoplasts prepared from epithelial cells, MTs displayed dynamic instability at plus ends and relative stability at minus ends presumably because of specific minus-end stability factors distributed throughout the cytoplasm. We suggest that, in fibroblast cells, a minus-end depolymerization mechanism functions to eliminate errors in MT organization and that dynamic instability of MT plus ends is a result of capping of minus ends by the centrosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.