ArticleProceedings of the National Academy of Sciences of the United States of America1999
A change in actin conformation associated with filament instability after Pi release.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
72 citing papers in PubMed, 161 citations in OpenAlex.
- Cooperative and divergent properties of bacterial actin isoforms inBiophysics and physicobiology · 2026Article
- High-resolution yeast actin structures indicate the molecular mechanism of actin filament stiffening by cations.Communications chemistry · 2024Article
- Article
- Structural Significance of His73 in F-Actin Dynamics: Insights from Ab Initio Study.International journal of molecular sciences · 2022Article
- Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nucleotide-dependent conformational changes in the actin filament: Subtler than expected.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells.Molecules (Basel, Switzerland) · 2018Article
- Long Noncoding RNA CRYBG3 Blocks Cytokinesis by Directly Binding G-Actin.Cancer research · 2018Article
- Apicomplexan actin polymerization depends on nucleation.Scientific reports · 2017Article
- Actin dynamics and cofilin-actin rods in alzheimer disease.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Actin age orchestrates myosin-5 and myosin-6 run lengths.Current biology : CB · 2015Article
- Near-atomic resolution for one state of F-actin.Structure (London, England : 1993) · 2015Article
- Damage of hippocampal neurons in rats with chronic alcoholism.Neural regeneration research · 2014Article
- Nucleotide regulation of the structure and dynamics of G-actin.Biophysical journal · 2014Article
- Mechanisms of nuclear actin in chromatin-remodeling complexes.Trends in cell biology · 2014Review
- Review
- ATP and ADP actin states.Biopolymers · 2013Article
- Structural states and dynamics of the D-loop in actin.Biophysical journal · 2012Article
- G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.The Journal of biological chemistry · 2012Article
- Building distinct actin filament networks in a common cytoplasm.Current biology : CB · 2011Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability of actin to both polymerize into filaments and to depolymerize permits the rapid rearrangements of actin structures that are essential for actin's function in most cellular processes. Filament polarity and dynamic properties are conferred by the hydrolysis of ATP on actin filaments. Release of inorganic phosphate (Pi) from filaments after ATP hydrolysis promotes depolymerization. We identify a yeast actin mutation, Val-159 to Asn, which uncouples Pi release from the conformational change that results in filament destabilization. Three-dimensional reconstructions of electron micrographs reveal a conformational difference between ADP-Pi filaments and ADP filaments and show that ADP V159N filaments resemble ADP-Pi wild-type filaments. Crystal structures of mammalian beta-actin in which the nucleotide binding cleft is in the "open" and "closed" states can be used to model actin filaments in the ADP and ADP-Pi conformations, respectively. We propose that these two conformations of G-actin may be related to two functional states of F-actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.