ArticleThe Journal of general physiology1999
Gating of single N-type calcium channels recorded from bullfrog sympathetic neurons.
Article in The Journal of general physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Neuronal junctophilins recruit specific CaeLife · 2021Article
- Buffer mobility and the regulation of neuronal calcium domains.Frontiers in cellular neuroscience · 2015Review
- Single channel measurements demonstrate the voltage dependence of permeation through N-type and L-type CaV channels.Channels (Austin, Tex.) · 2015Article
- Domain III regulates N-type (CaV2.2) calcium channel closing kinetics.Journal of neurophysiology · 2012Article
- Bile Acid Inhibition of N-type Calcium Channel Currents from Sympathetic Ganglion Neurons.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2012Article
- (R)-roscovitine prolongs the mean open time of unitary N-type calcium channel currents.Neuroscience · 2010Article
- Open-state occupancy prevents gating charge relaxation of N-type (CaV2.2) calcium channels.Biophysical journal · 2009Article
- Nanodomains of single Ca2+ channels contribute to action potential repolarization in cortical neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Alternative splicing in the C-terminus of CaV2.2 controls expression and gating of N-type calcium channels.The Journal of physiology · 2006Article
- Caveolin-1 expression and membrane cholesterol content modulate N-type calcium channel activity in NG108-15 cells.Biophysical journal · 2005Article
- Slowed N-type calcium channel (CaV2.2) deactivation by the cyclin-dependent kinase inhibitor roscovitine.Biophysical journal · 2005Article
- L-type calcium channel gating is modulated by bradykinin with a PKC-dependent mechanism in NG108-15 cells.European biophysics journal : EBJ · 2005Article
- A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients.Biophysical journal · 2005Article
- Endogenous Ca2+ buffer concentration and Ca2+ microdomains in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Modal gating of human CaV2.1 (P/Q-type) calcium channels: II. the b mode and reversible uncoupling of inactivation.The Journal of general physiology · 2004Article
- Neurotransmitter modulation of neuronal calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- Short-term synaptic plasticity, simulation of nerve terminal dynamics, and the effects of protein kinase C activation in rat hippocampus.The Journal of physiology · 2002Article
- Direct measurement of single-channel Ca(2+) currents in bullfrog hair cells reveals two distinct channel subtypes.The Journal of physiology · 2001Article
- Direct autocrine inhibition and cAMP-dependent potentiation of single L-type Ca2+ channels in bovine chromaffin cells.The Journal of physiology · 2001Article
- Reluctant gating of single N-type calcium channels during neurotransmitter-induced inhibition in bullfrog sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
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2 authors.
Funding
Abstract
For many neurons, N-type calcium channels provide the primary pathway for calcium influx during an action potential. We investigated the gating properties of single N-type calcium channels using the cell-attached patch technique. With 100 mM Ba2+ in the pipet, mean N-channel open probability (Po, measured over 100 ms) increased with depolarization, but the range at a single voltage was large (e.g., Po at +40 mV ranged from 0.1 to 0.8). The open dwell time histograms were generally well fit by a single exponential with mean open time (tauo) increasing from 0.7 ms at +10 mV to 3.1 ms at +40 mV. Shut time histograms were well fit by two exponentials. The brief shut time component (taush1 = 0.3 ms) did not vary with the test potential, while the longer shut time component (taush2) decreased with voltage from 18.9 ms at +10 mV to 2.3 ms at +40 mV. Although N-channel Po during individual sweeps at +40 mV was often high ( approximately 0.8), mean Po was reduced by null sweeps, low Po gating, inactivation, and slow activation. The variability in mean Po across patches resulted from differences in the frequency these different gating processes were expressed by the channels. Runs analysis showed that null sweeps tended to be clustered in most patches, but that inactivating and slowly activating sweeps were generally distributed randomly. Low Po gating (Po = 0.2, tauo = 1 ms at +40 mV) could be sustained for approximately 1 min in some patches. The clustering of null sweeps and sweeps with low Po gating is consistent with the idea that they result from different modes of N-channel gating. While Po of the main N-channel gating state is high, the net Po is reduced to a maximum value of close to 0.5 by other gating processes.
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