ArticleGenetics1999
Saccharomyces cerevisiae Mod5p-II contains sequences antagonistic for nuclear and cytosolic locations.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 55 citations in OpenAlex.
- tRNA hypomodification facilitates 5-fluorocytosine resistance via cross-pathway control system activation in Aspergillus fumigatus.Nucleic acids research · 2025Article
- Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase: Specificity determinants and tRNA-i6A37 profiles.PLoS genetics · 2020Article
- Cytokinins in Dictyostelia - A Unique Model for Studying the Functions of Signaling Agents From Species to Kingdoms.Frontiers in cell and developmental biology · 2020Review
- RNA polymerase II (RNAP II)-associated factors are recruited to tRNA loci, revealing that RNAP II- and RNAP III-mediated transcriptions overlap in yeast.The Journal of biological chemistry · 2019Article
- Phytohormone metabolism in human cells: Cytokinins are taken up and interconverted in HeLa cell culture.FASEB bioAdvances · 2019Article
- The role of intracellular compartmentalization on tRNA processing and modification.RNA biology · 2018Review
- The modified base isopentenyladenosine and its derivatives in tRNA.RNA biology · 2017Review
- Aggregation of Mod5 is affected by tRNA binding with implications for tRNA gene-mediated silencing.FEBS letters · 2017Article
- Lack of tRNA-i6A modification causes mitochondrial-like metabolic deficiency in S. pombe by limiting activity of cytosolic tRNATyr, not mito-tRNA.RNA (New York, N.Y.) · 2016Article
- Review
- Separate responses of karyopherins to glucose and amino acid availability regulate nucleocytoplasmic transport.Molecular biology of the cell · 2014Article
- Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA.PLoS genetics · 2014Article
- Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Lack of tRNA modification isopentenyl-A37 alters mRNA decoding and causes metabolic deficiencies in fission yeast.Molecular and cellular biology · 2013Article
- Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- tRNA biology charges to the front.Genes & development · 2010Review
- Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae.Molecular biology of the cell · 2010Article
- Review
- Mechanism and a peptide motif for targeting peripheral proteins to the yeast inner nuclear membrane.Traffic (Copenhagen, Denmark) · 2009Article
- Importin-beta family members mediate alpharetrovirus gag nuclear entry via interactions with matrix and nucleocapsid.Journal of virology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
MOD5 encodes a tRNA modification activity located in three subcellular compartments. Alternative translation initiation generates Mod5p-I, located in the mitochondria and the cytosol, and Mod5p-II, located in the cytosol and nucleus. Here we study the nucleus/cytosol distribution of overexpressed Mod5p-II. Nuclear Mod5p-II appears concentrated in the nucleolus, perhaps indicating that the nuclear pool may have a different biological role than the cytoplasmic and mitochondrial pools. Mod5p contains three motifs resembling bipartite-like nuclear localization sequences (NLSs), but only one is sufficient to locate a passenger protein to the nucleus. Mutations of basic residues of this motif cumulatively contribute to a cytosolic location for the fusion proteins. These alterations also cause decreased nuclear pools of endogenous Mod5p-II. Depletion of nuclear Mod5p-II does not affect tRNATyr function. Despite the NLS, most Mod5p is cytosolic. We assessed whether Mod5p sequences cause a karyophilic reporter to be located in the cytosol. By this assay, Mod5p may contain more than one region that functions as cytoplasmic retention and/or nuclear export sequences. Thus, distribution of Mod5p results from the presence/absence of mitochondrial targeting information and sequences antagonistic for nuclear and cytosolic locations. Mod5p is highly conserved; sequences responsible for subcellular distribution appear to reside in "accessory" motifs missing from prokaryotic counterparts.
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Registered trials
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