ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
79 citing papers in PubMed, 509 citations in OpenAlex.
- From the plate to the brain: associations between dietary patterns and reduced dementia prevalence and white matter lesions in older Japanese adults.GeroScience · 2026Article
- Looking for answers far away from the soma-the (un)known axonal functions of TDP-43, and their contribution to early NMJ disruption in ALS.Molecular neurodegeneration · 2023Review
- Unrestrained growth of correctly oriented microtubules instructs axonal microtubule orientation.eLife · 2022Article
- Axonal mRNA translation in neurological disorders.RNA biology · 2021Review
- Local gene regulation in radial glia: Lessons from across the nervous system.Traffic (Copenhagen, Denmark) · 2020Review
- Axonal precursor miRNAs hitchhike on endosomes and locally regulate the development of neural circuits.The EMBO journal · 2020Article
- mRNA localization and local translation in neurons.AIMS neuroscience · 2020Review
- Local translation in neurons: visualization and function.Nature structural & molecular biology · 2019Review
- Miles to go (mtgo) encodes FNDC3 proteins that interact with the chaperonin subunit CCT3 and are required for NMJ branching and growth in Drosophila.Developmental biology · 2019Article
- The Role of Axon Transport in Neuroprotection and Regeneration.Developmental neurobiology · 2018Review
- Article
- Expanding Axonal Transcriptome Brings New Functions for Axonally Synthesized Proteins in Health and Disease.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2018Review
- To the end of the line: Axonal mRNA transport and local translation in health and neurodegenerative disease.Developmental neurobiology · 2018Review
- Beneficial Effects of Resveratrol-Mediated Inhibition of the mTOR Pathway in Spinal Cord Injury.Neural plasticity · 2018Review
- Article
- mTOR regulates peripheral nerve response to tensile strain.Journal of neurophysiology · 2017Article
- Local translation in neuronal compartments: how local is local?EMBO reports · 2017Review
- Messenger RNAs localized to distal projections of human stem cell derived neurons.Scientific reports · 2017Article
- Accumulating Evidence for Axonal Translation in Neuronal Homeostasis.Frontiers in neuroscience · 2017Review
- Intra-axonal protein synthesis in development and beyond.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2016Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proteins needed for growth and maintenance of the axon are generally believed to be synthesized in the cell bodies and delivered to the axons by anterograde transport. However, recent reports suggest that some proteins can also be synthesized within axons. We used [35S]methionine metabolic labeling to investigate axonal protein synthesis in compartmented cultures of sympathetic neurons from newborn rats. Incubation of distal axons for 4 hr with [35S]methionine resulted in a highly specific pattern of labeled axonal proteins on SDS-PAGE, with 4 prominent bands in the 43-55 kDa range. The labeled proteins in axons were not synthesized in the cell bodies, because they were also produced by axons after the cell bodies had been removed. Two of the proteins were identified by immunoprecipitation as actin and beta-tubulin. Axons synthesized <1% of the actin and tubulin synthesized in the cell bodies and transported into the axons, and 75-85% inhibition of axonal protein synthesis by cycloheximide and puromycin failed to inhibit axonal elongation. Nonetheless, the specific production by axons of the major proteins of the axonal cytoskeleton suggests that axonal protein synthesis arises from specific mechanisms and likely has biological significance. One hypothetical scenario involves neurons with long axons in vivo in which losses from turnover during axonal transport may limit the availability of cell body synthesized proteins to the distal axons. In this case, a significant fraction of axonal proteins might be supplied by axonal synthesis, which could, therefore, play important roles in axonal maintenance, regeneration, and sprouting.
Indexed as
Identifiers
9870932PMC6782370W1538641465What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.