ArticleIn vitro cellular & developmental biology. Animal
Development of a serum-free medium for the production of humanized antibody from Chinese hamster ovary cells using a statistical design.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Review
- Development of a modified serum-free medium for Vero cell cultures: effects of protein hydrolysates, l-glutamine and SITE liquid media supplement on cell growth.Cytotechnology · 2021Article
- One-Time Optimization of Advanced T Cell Culture Media Using a Machine Learning Pipeline.Frontiers in bioengineering and biotechnology · 2021Article
- Benchmarking of commercially available CHO cell culture media for antibody production.Applied microbiology and biotechnology · 2015Article
- Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclonal Antibody.PloS one · 2015Article
- Serum-free media for the production of human mesenchymal stromal cells: a review.Cell proliferation · 2013Review
- Cell culture medium improvement by rigorous shuffling of components using media blending.Cytotechnology · 2013Article
- Effects of supplementation of various medium components on chinese hamster ovary cell cultures producing recombinant antibody.Cytotechnology · 2005Article
- Development of a serum-free medium for dihydrofolate reductase-deficient Chinese hamster ovary cells (DG44) using a statistical design: beneficial effect of weaning of cells.In vitro cellular & developmental biology. Animal · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To develop serum-free (SF) media for the production of humanized antibody from recombinant Chinese hamster ovary (rCHO) cells, a statistical optimization approach based on a Plackett-Burman design was adopted. A basal medium was prepared by supplementing alpha-minimal essential medium (alpha-MEM) with Fe(NO3)3.9H2O, CuCl2, ZnSO4.7H2O, and Na2SeO3 which are generally contained in SF medium formulations. Insulin, transferrin, and ethanolamine were also supplemented to the basal medium to determine their optimal concentrations. From this statistical analysis, serine, phenylalanine, and tyrosine were identified as important determinants for cell growth. Also, putrescine, linoleic acid, and hydrocortisone were shown to be important for both cell growth and antibody production. The SF medium was formulated by supplementing the basal medium with components showing positive effects on cell growth and/or antibody production. Cell growth and antibody production in this SF medium were comparable to those in alpha-MEM supplemented with 5% dialyzed fetal bovine serum. Taken together, the results obtained here show that a Plackett-Burman design facilitates the development of SF media for rCHO cells aimed at producing a humanized antibody.
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