ArticlePlant molecular biology1998
Characterization of a germin-like protein gene expressed in somatic and zygotic embryos of pine (Pinus caribaea Morelet).
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 58 citations in OpenAlex.
- Understanding the transition from embryogenesis to seed filling inFrontiers in plant science · 2025Article
- Transcriptomic, Metabolomic, and Physiological Analyses Reveal That the Culture Temperatures Modulate the Cryotolerance and Embryogenicity of Developing Somatic Embryos inFrontiers in plant science · 2021Article
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- iTRAQ-Based Quantitative Proteomic Analysis of Embryogenic and Non-embryogenic Calli Derived from a Maize (International journal of molecular sciences · 2018Article
- Versatility of germin-like proteins in their sequences, expressions, and functions.Functional & integrative genomics · 2015Article
- Do Cupins Have a Function Beyond Being Seed Storage Proteins?Frontiers in plant science · 2015Article
- Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.Molecular biology reports · 2013Article
- The molecular basis for stress-induced acquisition of somatic embryogenesis.Molecular biology reports · 2010Review
- Molecular aspects of somatic-to-embryogenic transition in plants.Journal of chemical biology · 2009Article
- Molecular regulation of somatic embryogenesis in potato: an auxin led perspective.Plant molecular biology · 2008Article
- Germin-like genes are expressed during somatic embryogenesis and early development of conifers.Plant molecular biology · 2006Article
- Germin-like protein gene family of a moss, Physcomitrella patens, phylogenetically falls into two characteristic new clades.Plant molecular biology · 2004Article
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- Identification of differentially expressed cDNA sequences and histological characteristics of Hevea brasiliensis calli in relation to their embryogenic and regenerative capacities.Plant cell reports · 2004Article
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- Use of serial analysis of gene expression technology to reveal changes in gene expression in Arabidopsis pollen undergoing cold stress.Plant physiology · 2003Article
- Microbial relatives of the seed storage proteins of higher plants: conservation of structure and diversification of function during evolution of the cupin superfamily.Microbiology and molecular biology reviews : MMBR · 2000Review
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7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germin-like proteins (GLPs) ionically bound to the walls of preglobular somatic embryos of Pinus caribaea Morelet are markers of this early developmental stage. In order to reveal the physiological implications of such markers during early embryo development, we isolated a cDNA clone from somatic embryos predicted to encode a protein with sequence similarity to GLPs. PcGER1 has an open reading frame corresponding to a 220 amino acid polypeptide with a putative N-glycosylation site on Asn-69. The presence of a 24 amino acid putative signal peptide supports the hypothesis of an apoplastic location. The N-terminal 20 amino acid sequence of the predicted mature protein is identical to the amino terminal sequence of GP111, one of the extracellular pine GLPs previously identified. Southern blot hybridizations indicate that PcGER1 is probably unique in the pine genome. Transcripts homologous to PcGER1 are abundant in all embryogenic lines, absent from nonembryogenic lines, and present in quiescent zygotic embryos but not in the female gametophyte, the haploid storage tissue of conifers. Their abundance sharply decreases during germination. Isolation of gf-0.8, a genomic fragment identical to PcGER1 cDNA sequence, confirms that no introns disrupt the coding region as it has been already described for wheat gf-2.8 and gf-3.8 genomic clones. Recombinant PcGER1, produced in Escherichia coli, is recognized by antibodies raised against the GP111 N-terminal nonapeptide and the unglycosylated wheat germin monomer. The implications of GLPs in pine embryogenesis are discussed.
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