ArticleThe Journal of biological chemistry1999
Intracellular glycerol levels modulate the activity of Sln1p, a Saccharomyces cerevisiae two-component regulator.
Article in The Journal of biological chemistry, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield.Applied microbiology and biotechnology · 2018Article
- The yeasts phosphorelay systems: a comparative view.World journal of microbiology & biotechnology · 2017Review
- Article
- Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress.International journal of molecular sciences · 2016Article
- Complex regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA in Saccharomyces cerevisiae: experimental and computational evidences.BMC systems biology · 2015Article
- Deletion of FPS1, encoding aquaglyceroporin Fps1p, improves xylose fermentation by engineered Saccharomyces cerevisiae.Applied and environmental microbiology · 2013Article
- Current understanding of HOG-MAPK pathway in Aspergillus fumigatus.Mycopathologia · 2013Review
- An integrated pathway system modeling of Saccharomyces cerevisiae HOG pathway: a Petri net based approach.Molecular biology reports · 2013Article
- Response to hyperosmotic stress.Genetics · 2012Review
- Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress.BMC biology · 2012Article
- Stress Response and Pathogenicity of the Necrotrophic Fungal Pathogen Alternaria alternata.Scientifica · 2012Review
- Association of the Skn7 and Yap1 transcription factors in the Saccharomyces cerevisiae oxidative stress response.Eukaryotic cell · 2011Article
- Fungal Skn7 stress responses and their relationship to virulence.Eukaryotic cell · 2011Review
- Genetic and biochemical analysis of the SLN1 pathway in Saccharomyces cerevisiae.Methods in enzymology · 2010Article
- Effects of osmolytes on the SLN1-YPD1-SSK1 phosphorelay system from Saccharomyces cerevisiae.Biochemistry · 2009Article
- Master and commander in fungal pathogens: the two-component system and the HOG signaling pathway.Eukaryotic cell · 2008Review
- Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein.The Journal of biological chemistry · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The HOG mitogen-activated protein kinase pathway mediates the osmotic stress response in Saccharomyces cerevisiae, activating genes like GPD1 (glycerol phosphate dehydrogenase), required for survival under hyperosmotic conditions. Activity of this pathway is regulated by Sln1p, a homolog of the "two-component" histidine kinase family of signal transduction molecules prominent in bacteria. Sln1p also regulates the activity of a Hog1p-independent pathway whose transcriptional output can be monitored using an Mcm1p-dependent lacZ reporter gene. The relationship between the two Sln1p branches is unclear, however, the requirement for unphosphorylated pathway intermediates in Hog1p pathway activation and for phosphorylated intermediates in the activation of the Mcm1p reporter suggests that the two Sln1p branches are reciprocally regulated. To further investigate the signals and molecules involved in modulating Sln1p activity, we have screened for new mutations that elevate the activity of the Mcm1p-dependent lacZ reporter gene. We find that loss of function mutations in FPS1, a gene encoding the major glycerol transporter in yeast activates the reporter in a SLN1-dependent fashion. We propose that elevated intracellular glycerol levels in the fps1 mutant shift Sln1p to the phosphorylated state and trigger the Sln1-dependent activity of the Mcm1 reporter. These observations are consistent with a model in which Sln1p autophosphorylation is triggered by a hypo-osmotic stimulus and indicate that the Sln1p osmosensor is tied generally to osmotic balance, and may not specifically sense an external osmolyte.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.