ArticleThe Journal of cell biology1998
Nonuniform microtubular polarity established by CHO1/MKLP1 motor protein is necessary for process formation of podocytes.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- The Life of a Kidney Podocyte.Acta physiologica (Oxford, England) · 2025Review
- Cytoskeleton-associated protein 4 affects podocyte cytoskeleton dynamics in diabetic kidney disease.JCI insight · 2025Article
- The midbody and midbody remnant: from cellular debris to signaling organelle with diagnostic and therapeutic potential.Molecular biology of the cell · 2025Review
- Genetics and phenotypic heterogeneity of Galloway-Mowat syndrome.Cell communication and signaling : CCS · 2025Review
- Dysregulated Dynein-Mediated Trafficking of Nephrin Causes INF2-related Podocytopathy.Journal of the American Society of Nephrology : JASN · 2021Article
- Centromere protein I (CENPI) is a candidate gene for X-linked steroid sensitive nephrotic syndrome.Journal of nephrology · 2020Article
- Networks that link cytoskeletal regulators and diaphragm proteins underpin filtration function in Drosophila nephrocytes.Experimental cell research · 2018Article
- High-content screening assay-based discovery of paullones as novel podocyte-protective agents.American journal of physiology. Renal physiology · 2018Article
- The Evolving Complexity of the Podocyte Cytoskeleton.Journal of the American Society of Nephrology : JASN · 2017Review
- From tubular sublimate nephropathy via urinary concentrating mechanism to glomerular disease-Wilhelm Kriz's contribution to modern nephrology : On the occasion of a symposium honoring Wilhelm Kriz on his 80th birthday.Pflugers Archiv : European journal of physiology · 2017Article
- A small molecule screening to detect potential therapeutic targets in human podocytes.American journal of physiology. Renal physiology · 2017Article
- RNA sequencing analysis of human podocytes reveals glucocorticoid regulated gene networks targeting non-immune pathways.Scientific reports · 2016Article
- Article
- Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression.PloS one · 2015Article
- Loss-of-function mutations in WDR73 are responsible for microcephaly and steroid-resistant nephrotic syndrome: Galloway-Mowat syndrome.American journal of human genetics · 2014Article
- The podocyte cytoskeleton--key to a functioning glomerulus in health and disease.Nature reviews. Nephrology · 2011Review
- Marker expression, behaviors, and responses vary in different lines of conditionally immortalized cultured podocytes.American journal of physiology. Renal physiology · 2011Article
- Hooks and comets: The story of microtubule polarity orientation in the neuron.Developmental neurobiology · 2011Review
- Identification of proteins that interact with podocin using the yeast 2-hybrid system.Yonsei medical journal · 2009Article
- Nuclear relocation of the nephrin and CD2AP-binding protein dendrin promotes apoptosis of podocytes.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Podocytes are unique cells that are decisively involved in glomerular filtration. They are equipped with a complex process system consisting of major processes and foot processes whose function is insufficiently understood (Mundel, P., and W. Kriz. 1995. Anat. Embryol. 192:385-397). The major processes of podocytes contain a microtubular cytoskeleton. Taking advantage of a recently established cell culture system for podocytes with preserved ability to form processes (Mundel, P., J. Reiser, A. Zúñiga Mejía Borja, H. Pavenstädt, G.R. Davidson, W. Kriz, and R. Zeller. 1997b. Exp. Cell Res. 36:248-258), we studied the functional significance of the microtubular system in major processes. The following data were obtained: (a) Microtubules (MTs) in podocytes show a nonuniform polarity as revealed by hook-decoration. (b) CHO1/ MKLP1, a kinesin-like motor protein, is associated with MTs in podocytes. (c) Treatment of differentiating podocytes with CHO1/MKLP1 antisense oligonucleotides abolished the formation of processes and the nonuniform polarity of MTs. (d) During the recovery from taxol treatment, taxol-stabilized (nocodazole- resistant) MT fragments were distributed in the cell periphery along newly assembled nocodazole-sensitive MTs. A similar distribution pattern of CHO1/MKLP1 was found under these circumstances, indicating its association with MTs. (e) In the recovery phase after complete depolymerization, MTs reassembled exclusively at centrosomes. Taken together, these findings lead to the conclusion that the nonuniform MT polarity in podocytes established by CHO1/MKLP1 is necessary for process formation.
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