ArticleThe Journal of cell biology1998
Biochemical and functional studies of cortical vesicle fusion: the SNARE complex and Ca2+ sensitivity.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 139 citations in OpenAlex.
- Proteomics-The State of the Field: The Definition and Analysis of Proteomes Should Be Based in Reality, Not Convenience.Proteomes · 2024Article
- Hypothesis Relating the Structure, Biochemistry and Function of Active Zone Material Macromolecules at a Neuromuscular Junction.Frontiers in synaptic neuroscience · 2021Article
- Unbiased Thiol-Labeling and Top-Down Proteomic Analyses Implicate Multiple Proteins in the Late Steps of Regulated Secretion.Proteomes · 2019Article
- Article
- Changes to the Human Serum Proteome in Response to High Intensity Interval Exercise: A Sequential Top-Down Proteomic Analysis.Frontiers in physiology · 2019Article
- Lipid-dependence of target membrane stability during influenza viral fusion.Journal of cell science · 2018Article
- Application of High-Throughput Assays to Examine Phospho-Modulation of the Late Steps of Regulated Exocytosis.High-throughput · 2017Article
- The Role of Phospholipase D in Regulated Exocytosis.The Journal of biological chemistry · 2015Article
- Quantification of exocytosis kinetics by DIC image analysis of cortical lawns.Journal of chemical biology · 2014Article
- Coomassie blue as a near-infrared fluorescent stain: a systematic comparison with Sypro Ruby for in-gel protein detection.Molecular & cellular proteomics : MCP · 2013Article
- Function suggests nano-structure: towards a unified theory for secretion rate, a statistical mechanics approach.Journal of the Royal Society, Interface · 2013Article
- A new approach to the molecular analysis of docking, priming, and regulated membrane fusion.Journal of chemical biology · 2011Article
- Cell surface changes in the egg at fertilization.Molecular reproduction and development · 2009Review
- Roles of cholesterol in vesicle fusion and motion.Biophysical journal · 2009Article
- Model of SNARE-mediated membrane adhesion kinetics.PloS one · 2009Article
- Enhancement of the Ca(2+)-triggering steps of native membrane fusion via thiol-reactivity.Journal of chemical biology · 2009Article
- Copper (II) sulfate charring for high sensitivity on-plate fluorescent detection of lipids and sterols: quantitative analyses of the composition of functional secretory vesicles.Journal of chemical biology · 2008Article
- Specific lipids supply critical negative spontaneous curvature--an essential component of native Ca2+-triggered membrane fusion.Biophysical journal · 2008Article
- Motion matters: secretory granule motion adjacent to the plasma membrane and exocytosis.Molecular biology of the cell · 2006Article
- Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis.Proteome science · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cortical vesicles (CV) possess components critical to the mechanism of exocytosis. The homotypic fusion of CV centrifuged or settled into contact has a sigmoidal Ca2+ activity curve comparable to exocytosis (CV-PM fusion). Here we show that Sr2+ and Ba2+ also trigger CV-CV fusion, and agents affecting different steps of exocytotic fusion block Ca2+, Sr2+, and Ba2+-triggered CV-CV fusion. The maximal number of active fusion complexes per vesicle, <n\>Max, was quantified by NEM inhibition of fusion, showing that CV-CV fusion satisfies many criteria of a mathematical analysis developed for exocytosis. Both <n\>Max and the Ca2+ sensitivity of fusion complex activation were comparable to that determined for CV-PM fusion. Using Ca2+-induced SNARE complex disruption, we have analyzed the relationship between membrane fusion (CV-CV and CV-PM) and the SNARE complex. Fusion and complex disruption have different sensitivities to Ca2+, Sr2+, and Ba2+, the complex remains Ca2+- sensitive on fusion-incompetent CV, and disruption does not correlate with the quantified activation of fusion complexes. Under conditions which disrupt the SNARE complex, CV on the PM remain docked and fusion competent, and isolated CV still dock and fuse, but with a markedly reduced Ca2+ sensitivity. Thus, in this system, neither the formation, presence, nor disruption of the SNARE complex is essential to the Ca2+-triggered fusion of exocytotic membranes. Therefore the SNARE complex alone cannot be the universal minimal fusion machine for intracellular fusion. We suggest that this complex modulates the Ca2+ sensitivity of fusion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.