ArticleJournal of bacteriology1999
Pkg2, a novel transmembrane protein Ser/Thr kinase of Streptomyces granaticolor.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss.BMC complementary and alternative medicine · 2019Article
- The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of ZoonoticFrontiers in cellular and infection microbiology · 2017Article
- A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence.BMC genomics · 2011Article
- Eukaryote-like serine/threonine kinases and phosphatases in bacteria.Microbiology and molecular biology reviews : MMBR · 2011Review
- Article
- General and molecular microbiology and microbial genetics in the IM CAS.Journal of industrial microbiology & biotechnology · 2010Review
- Comparative study of the life cycle dependent post-translation modifications of protein synthesis elongation factor Tu present in the membrane proteome of streptomycetes and mycobacteria.Folia microbiologica · 2010Article
- The sole serine/threonine protein kinase and its cognate phosphatase from Aquifex aeolicus targets pyrimidine biosynthesis.Molecular and cellular biochemistry · 2008Article
- Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae.Journal of bacteriology · 2007Article
- Effect of protein kinase inhibitors on protein phosphorylation and germination of aerial spores from Streptomyces coelicolor.Folia microbiologica · 2007Article
- Diversity in domain architectures of Ser/Thr kinases and their homologues in prokaryotes.BMC genomics · 2005Article
- Role of Mycobacterium tuberculosis Ser/Thr kinase PknF: implications in glucose transport and cell division.Journal of bacteriology · 2005Article
- Effect of phosphate on the expression of protein-Ser/Thr kinase pkg2 in Streptomyces granaticolor.Folia microbiologica · 2005Article
- Identification of two eukaryote-like serine/threonine kinases encoded by Chlamydia trachomatis serovar L2 and characterization of interacting partners of Pkn1.Infection and immunity · 2003Article
- Autophosphorylation of a bacterial serine/threonine kinase, AfsK, is inhibited by KbpA, an AfsK-binding protein.Journal of bacteriology · 2001Article
- Evidence for phosphoprotein phosphatase in Streptomyces granaticolor.Folia microbiologica · 2000Article
- Expression and characterization of the Mycobacterium tuberculosis serine/threonine protein kinase PknB.Infection and immunity · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 4.2-kb SphI-BamHI fragment of chromosomal DNA from Streptomyces granaticolor was cloned and shown to encode a protein with significant sequence similarity to the eukaryotic protein serine/threonine kinases. It consists of 701 amino acids and in the N-terminal part contains all conserved catalytic domains of protein kinases. The C-terminal domain of Pkg2 contains seven tandem repeats of 11 or 12 amino acids with similarity to the tryptophan-docking motif known to stabilize a symmetrical three-dimensional structure called a propeller structure. The pkg2 gene was overexpressed in Escherichia coli, and the gene product (Pkg2) has been found to be autophosphorylated at serine and threonine residues. The N- and C-terminal parts of Pkg2 are separated with a hydrophobic stretch of 21 amino acids which translocated a PhoA fusion protein into the periplasm. Thus, Pkg2 is the first transmembrane protein serine/threonine kinase described for streptomycetes. Replacement of the pkg2 gene by the spectinomycin resistance gene resulted in changes in the morphology of aerial hyphae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.