ArticleInfection and immunity1999
Establishment of unipolar localization of IcsA in Shigella flexneri 2a is not dependent on virulence plasmid determinants.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Human guanylate binding proteins: nanomachines orchestrating host defense.The FEBS journal · 2021Review
- Biogenesis of YidC cytoplasmic membrane substrates is required for positioning of autotransporter IcsA at future poles.Journal of bacteriology · 2014Article
- Surface display of the HPV L1 capsid protein by the autotransporter Shigella IcsA.Journal of microbiology (Seoul, Korea) · 2014Article
- Article
- A genome-scale proteomic screen identifies a role for DnaK in chaperoning of polar autotransporters in Shigella.Journal of bacteriology · 2009Article
- Shigella flexneri inhibits staurosporine-induced apoptosis in epithelial cells.Infection and immunity · 2007Article
- The bacterial actin-like cytoskeleton.Microbiology and molecular biology reviews : MMBR · 2006Review
- Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of bacteriology · 2006Article
- Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB.Journal of bacteriology · 2005Article
- Branching sites and morphological abnormalities behave as ectopic poles in shape-defective Escherichia coli.Molecular microbiology · 2004Article
- Regulation of IcsP, the outer membrane protease of the Shigella actin tail assembly protein IcsA, by virulence plasmid regulators VirF and VirB.Journal of bacteriology · 2004Article
- Evidence for polar positional information independent of cell division and nucleoid occlusion.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Shigella flexneri DegP facilitates IcsA surface expression and is required for efficient intercellular spread.Infection and immunity · 2002Article
- MxiE regulates intracellular expression of factors secreted by the Shigella flexneri 2a type III secretion system.Journal of bacteriology · 2002Article
- dksA is required for intercellular spread of Shigella flexneri via an RpoS-independent mechanism.Infection and immunity · 2001Article
- Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Polarity in action: asymmetric protein localization in bacteria.Journal of bacteriology · 2001Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Unipolar localization of IcsA on the surface of Shigella flexneri is required for efficient formation of actin tails and protrusions in infected eucaryotic cells. Lipopolysaccharide (LPS) mutations have been demonstrated to affect either the establishment or the maintenance of IcsA in a unipolar location, although the mechanism is unknown. In order to analyze the contribution of virulence plasmid determinants on the unipolar localization of IcsA, we examined the localization of IcsA expressed from a cloned plasmid copy in two different genetic backgrounds. The localization of IcsA was first examined in a virulence plasmid-cured derivative of the wild-type S. flexneri 2a isolate 2457T. This approach examined the contribution of virulence plasmid-borne factors, including the previously identified virulence plasmid-borne protease that is responsible for cleaving IcsA in the outer membrane and releasing the 95-kDa secreted form from the cell surface. IcsA localization in a related but nonpathogenic Escherichia coli strain expressing LPS of the O8 serotype was also examined. IcsA surface presentation in both of these genetic backgrounds continued to be unipolar, demonstrating that virulence plasmid-borne determinants are not responsible for unipolar localization of IcsA. The unipolar localization of IcsA in the E. coli background suggests that a common pathway that allows IcsA to be spatially restricted to one pole on the bacterial cell surface exists in Shigella and E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.