ArticlePlant molecular biology1998
Chimeric calcium/calmodulin-dependent protein kinase in tobacco: differential regulation by calmodulin isoforms.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- Abundant protein phosphorylation potentially regulates Arabidopsis anther development.Journal of experimental botany · 2016Article
- Phylogeny of Plant Calcium and Calmodulin-Dependent Protein Kinases (CCaMKs) and Functional Analyses of Tomato CCaMK in Disease Resistance.Frontiers in plant science · 2015Article
- Abiotic stress responses in plants: roles of calmodulin-regulated proteins.Frontiers in plant science · 2015Review
- Article
- Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa).Molecular biology reports · 2013Article
- Rhizobial and fungal symbioses show different requirements for calmodulin binding to calcium calmodulin-dependent protein kinase in Lotus japonicus.The Plant cell · 2012Article
- Expression profile of calcium-dependent protein kinase (CDPKs) genes during the whole lifespan and under phytohormone treatment conditions in rice (Oryza sativa L. ssp. indica).Plant molecular biology · 2009Article
- Characterization of a calmodulin-regulated Ca2+-dependent-protein-kinase-related protein kinase, AtCRK1, from Arabidopsis.The Biochemical journal · 2004Article
- Characterization of a novel calcium/calmodulin-dependent protein kinase from tobacco.Plant physiology · 2004Article
- A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A tobacco (Nicotiana tabaccum) calmodulin-binding protein kinase, NtCBK2, is regulated differentially by calmodulin isoforms.The Biochemical journal · 2003Article
- Calcium in plants.Annals of botany · 2003Review
- The Arabidopsis CDPK-SnRK superfamily of protein kinases.Plant physiology · 2003Article
- Purification and characterization of a Ca(2+)-dependent/calmodulin-stimulated protein kinase from moss chloronema cells.Journal of biosciences · 2003Article
- Molecular and biochemical characterization of a calcium/calmodulin-binding protein kinase from rice.The Biochemical journal · 2002Article
- Differential messenger RNA gradients in the unicellular alga Acetabularia acetabulum. Role of the cytoskeleton.Plant physiology · 2002Article
- Calcium at the crossroads of signaling.The Plant cell · 2002Review
- Differential regulation of Ca2+/calmodulin-dependent enzymes by plant calmodulin isoforms and free Ca2+ concentration.The Biochemical journal · 2000Article
- Cloning and sequence analysis of lily and tobacco guanylate kinases.Molecular biology reports · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cDNA clones of chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) from tobacco (TCCaMK-1 and TCCaMK-2) were isolated and characterized. The polypeptides encoded by TCCaMK-1 and TCCaMK-2 have 15 different amino acid substitutions, yet they both contain a total of 517 amino acids. Northern analysis revealed that CCaMK is expressed in a stage-specific manner during anther development. Messenger RNA was detected when tobacco bud sizes were between 0.5 cm and 1.0 cm. The appearance of mRNA coincided with meiosis and became undetectable at later stages of anther development. The reverse polymerase chain reaction (RT-PCR) amplification assay using isoform-specific primers showed that both of the CCaMK mRNAs were expressed in anther with similar expression patterns. The CCaMK protein expressed in Escherichia coli showed Ca2+-dependent autophosphorylation and Ca2+/calmodulin-dependent substrate phosphorylation. Calmodulin isoforms (PCM1 and PCM6) had differential effects on the regulation of autophosphorylation and substrate phosphorylation of tobacco CCaMK, but not lily CCaMK. The evolutionary tree of plant serine/threonine protein kinases revealed that calmodulin-dependent kinases form one subgroup that is distinctly different from Ca2+-dependent protein kinases (CDPKs) and other serine/threonine kinases in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.