ArticleProceedings of the National Academy of Sciences of the United States of America1998
Real-time quantitative measurement of autocrine ligand binding indicates that autocrine loops are spatially localized.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor.Scientific reports · 2016Article
- The role of engineering approaches in analysing cancer invasion and metastasis.Nature reviews. Cancer · 2013Review
- Autocrine IFN-γ promotes naive CD8 T cell differentiation and synergizes with IFN-α to stimulate strong function.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Attenuation of extrinsic signaling reveals the importance of matrix remodeling on maintenance of embryonic stem cell self-renewal.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Subcellular resolution mapping of endogenous cytokine secretion by nano-plasmonic-resonator sensor array.Nano letters · 2011Article
- TNF-α-converting enzyme/a disintegrin and metalloprotease-17 mediates mechanotransduction in murine tracheal epithelial cells.American journal of respiratory cell and molecular biology · 2011Article
- An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Computational model and microfluidic platform for the investigation of paracrine and autocrine signaling in mouse embryonic stem cells.Molecular bioSystems · 2009Article
- From pathway to population--a multiscale model of juxtacrine EGFR-MAPK signalling.BMC systems biology · 2008Article
- Multiple mechanisms are responsible for transactivation of the epidermal growth factor receptor in mammary epithelial cells.The Journal of biological chemistry · 2008Article
- Paracrinicity: the story of 30 years of cellular pituitary crosstalk.Journal of neuroendocrinology · 2008Review
- Cell surface receptors for signal transduction and ligand transport: a design principles study.PLoS computational biology · 2007Review
- Computational modeling of extracellular mechanotransduction.Biophysical journal · 2006Article
- Cardiomyocyte hypertrophy and degradation of connexin43 through spatially restricted autocrine/paracrine heparin-binding EGF.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Ligand accumulation in autocrine cell cultures.Biophysical journal · 2005Article
- Article
- Self-organization of polarized cell signaling via autocrine circuits: computational model analysis.Biophysical journal · 2004Article
- Stochastic model of autocrine and paracrine signals in cell culture assays.Biophysical journal · 2003Article
- Spatial range of autocrine signaling: modeling and computational analysis.Biophysical journal · 2001Article
- IGF-I stimulation of extracellular acidification is not linked to cell proliferation for autocrine cells.Endocrine · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autocrine ligands are important regulators of many normal tissues and have been implicated in a number of disease states, including cancer. However, because by definition autocrine ligands are synthesized, secreted, and bound to cell receptors within an intrinsically self-contained "loop," standard pharmacological approaches cannot be used to investigate relationships between ligand/receptor binding and consequent cellular responses. We demonstrate here a new approach for measurement of autocrine ligand binding to cells, using a microphysiometer assay originally developed for investigating cell responses to exogenous ligands. This technique permits quantitative measurements of autocrine responses on the time scale of receptor binding and internalization, thus allowing investigation of the role of receptor trafficking and dynamics in cellular responses. We used this technique to investigate autocrine signaling through the epidermal growth factor receptor by transforming growth factor alpha (TGFalpha) and found that anti-receptor antibodies are far more effective than anti-ligand antibodies in inhibiting autocrine signaling. This result indicates that autocrine-based signals can operate in a spatially restricted, local manner and thus provide cells with information on their local microenvironment.
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