ArticleMolecular and cellular biology1999
Human osteogenesis involves differentiation-dependent increases in the morphogenically active 3' alternative splicing variant of acetylcholinesterase.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The significance of aryl acylamidase activity of acetylcholinesterase in osteoblast differentiation and mineralization.Molecular and cellular biochemistry · 2018Article
- Acetylcholinesterase plays a non-neuronal, non-esterase role in organogenesis.Development (Cambridge, England) · 2017Article
- Wnt3a induces the expression of acetylcholinesterase during osteoblast differentiation via the Runx2 transcription factor.The Journal of biological chemistry · 2017Article
- Endochondral Ossification Is Accelerated in Cholinesterase-Deficient Mice and in Avian Mesenchymal Micromass Cultures.PloS one · 2017Article
- Acetylcholinesterase Regulates Skeletal In Ovo Development of Chicken Limbs by ACh-Dependent and -Independent Mechanisms.PloS one · 2016Article
- Donepezil prevents RANK-induced bone loss via inhibition of osteoclast differentiation by downregulating acetylcholinesterase.Heliyon · 2015Article
- Simultaneous subchronic exposure to selenium and diazinon as possible risk factor for osteoporosis in adult male rats.Acta veterinaria Scandinavica · 2013Article
- Article
- Reduced bone formation in UK Gulf War veterans: a bone histomorphometric study.Journal of clinical pathology · 2002Article
- Changes in neuronal acetylcholinesterase gene expression and division of labor in honey bee colonies.Journal of molecular neuroscience : MN · 2001Article
- CREB regulates AChE-R-induced proliferation of human glioblastoma cells.Neoplasia (New York, N.Y.)Article
- Synaptogenesis and myopathy under acetylcholinesterase overexpression.Journal of molecular neuroscience : MNArticle
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8 authors.
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Abstract
The extended human acetylcholinesterase (AChE) promoter contains many binding sites for osteogenic factors, including 1,25-(OH)2 vitamin D3 and 17beta-estradiol. In differentiating osteosarcoma Saos-2 cells, both of these factors enhanced transcription of the AChE mRNA variant 3' terminated with exon 6 (E6-AChE mRNA), which encodes the catalytically and morphogenically active E6-AChE isoform. In contrast, antisense oligodeoxynucleotide suppression of E6-AChE mRNA expression increased Saos-2 proliferation in a dose- and sequence-dependent manner. The antisense mechanism of action was most likely mediated by mRNA destruction or translational arrest, as cytochemical staining revealed reduction in AChE gene expression. In vivo, we found that E6-AChE mRNA levels rose following midgestation in normally differentiating, postproliferative fetal chondrocytes but not in the osteogenically impaired chondrocytes of dwarf fetuses with thanatophoric dysplasia. Taken together, these findings suggest morphogenic involvement of E6-AChE in the proliferation-differentiation balance characteristic of human osteogenesis.
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