ArticleMolecular and cellular biology1999
Analysis of mice with single and multiple copies of transgenes reveals a novel arrangement for the lambda5-VpreB1 locus control region.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- The 3D Genome: From Structure to Function.International journal of molecular sciences · 2021Review
- Methods for mapping 3D chromosome architecture.Nature reviews. Genetics · 2020Review
- Three-dimensional genome architecture: players and mechanisms.Nature reviews. Molecular cell biology · 2015Review
- Transcription-coupled eviction of histones H2A/H2B governs V(D)J recombination.The EMBO journal · 2013Article
- Ectopic T cell receptor-α locus control region activity in B cells is suppressed by direct linkage to two flanking genes at once.PloS one · 2010Article
- Position effect variegation and imprinting of transgenes in lymphocytes.Nucleic acids research · 2008Article
- Gene regulation and large-scale chromatin organization in the nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2006Review
- Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division.EMBO reports · 2005Article
- Formation of an active tissue-specific chromatin domain initiated by epigenetic marking at the embryonic stem cell stage.Molecular and cellular biology · 2005Article
- Prospective estimation of recombination signal efficiency and identification of functional cryptic signals in the genome by statistical modeling.The Journal of experimental medicine · 2003Article
- Spatial organization of gene expression: the active chromatin hub.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Locus control regions.Blood · 2002Review
- Macrophage-specific gene expression: current paradigms and future challenges.International journal of hematology · 2002Review
- Binding of Ikaros to the lambda5 promoter silences transcription through a mechanism that does not require heterochromatin formation.The EMBO journal · 2001Article
- Bruton's tyrosine kinase regulates the activation of gene rearrangements at the lambda light chain locus in precursor B cells in the mouse.The Journal of experimental medicine · 2001Article
- Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development.Immunology · 2000Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The murine lambda5-VpreB1 locus encodes two proteins that form part of the pre-B-cell receptor and play a key role in B-lymphocyte development. We have identified a locus control region (LCR) which is responsible for coordinate activation of both genes in pre-B cells. Analysis of mice with single and multiple copies of transgenes shows a clear difference in the expression behavior of the genes depending on the transgene copy number. While expression of both lambda5 and VpreB1 in single- and two-copy integrations requires the presence of a set of DNase I hypersensitive sites located 3' of the lambda5 gene, small fragments containing the genes have LCR activity when arranged in multiple-copy tandem arrays, indicating that additional components of the LCR are located within or close to the genes. The complete LCR is capable of driving efficient copy-dependent expression of a lambda5 gene in pre-B cells even when it is integrated into centomeric gamma-satellite DNA. The finding that activation of expression of the locus by positively acting factors is fully dominant over the silencing effect of heterochromatin has implications for models for chromatin-mediated gene silencing during B-cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.