ArticleMolecular and cellular biology1999
Elevated levels of a U4/U6.U5 snRNP-associated protein, Spp381p, rescue a mutant defective in spliceosome maturation.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Dynamics and evolutionary conservation of B complex protein recruitment during spliceosome activation.Nucleic acids research · 2025Article
- Dynamics and Evolutionary Conservation of B Complex Protein Recruitment During Spliceosome Activation.bioRxiv : the preprint server for biology · 2024Article
- Recruitment of a splicing factor to the nuclear lamina for its inactivation.Communications biology · 2022Article
- Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability.Cell reports · 2019Article
- An Allosteric Network for Spliceosome Activation Revealed by High-Throughput Suppressor Analysis inGenetics · 2019Article
- Structure of a pre-catalytic spliceosome.Nature · 2017Article
- Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor.BMC evolutionary biology · 2017Article
- Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome.PeerJ · 2013Article
- Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1Δ cells.Nucleic acids research · 2011Article
- Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.Genetics · 2009Article
- Inhibition of a spliceosome turnover pathway suppresses splicing defects.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
- Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
U4 snRNA release from the spliceosome occurs through an essential but ill-defined Prp38p-dependent step. Here we report the results of a dosage suppressor screen to identify genes that contribute to PRP38 function. Elevated expression of a previously uncharacterized gene, SPP381, efficiently suppresses the growth and splicing defects of a temperature-sensitive (Ts) mutant prp38-1. This suppression is specific in that enhanced SPP381 expression does not alter the abundance of intronless RNA transcripts or suppress the Ts phenotypes of other prp mutants. Since SPP381 does not suppress a prp38::LEU2 null allele, it is clear that Spp381p assists Prp38p in splicing but does not substitute for it. Yeast SPP381 disruptants are severely growth impaired and accumulate unspliced pre-mRNA. Immune precipitation studies show that, like Prp38p, Spp381p is present in the U4/U6.U5 tri-snRNP particle. Two-hybrid analyses support the view that the carboxyl half of Spp381p directly interacts with the Prp38p protein. A putative PEST proteolysis domain within Spp381p is dispensable for the Spp381p-Prp38p interaction and for prp38-1 suppression but contributes to Spp381p function in splicing. Curiously, in vitro, Spp381p may not be needed for the chemistry of pre-mRNA splicing. Based on the in vivo and in vitro results presented here, we propose that two small acidic proteins without obvious RNA binding domains, Spp381p and Prp38p, act in concert to promote U4/U5.U6 tri-snRNP function in the spliceosome cycle.
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