ArticleMolecular and cellular biology1999
SL1 trans splicing and 3'-end formation in a novel class of Caenorhabditis elegans operon.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Genome annotation of Caenorhabditis briggsae by TEC-RED identifies new exons, paralogs, and conserved and novel operons.G3 (Bethesda, Md.) · 2022Article
- SLIDR and SLOPPR: flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data.BMC bioinformatics · 2021Article
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus.Journal of veterinary science · 2012Article
- A global analysis of C. elegans trans-splicing.Genome research · 2011Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- A novel heme-responsive element mediates transcriptional regulation in Caenorhabditis elegans.The Journal of biological chemistry · 2010Article
- Functional analysis of putative operons in Brugia malayi.International journal for parasitology · 2010Article
- Characterizing the transcriptional regulation of let-721, a Caenorhabditis elegans homolog of human electron flavoprotein dehydrogenase.Molecular genetics and genomics : MGG · 2009Article
- C. elegans sequences that control trans-splicing and operon pre-mRNA processing.RNA (New York, N.Y.) · 2007Article
- Genomic overview of mRNA 5'-leader trans-splicing in the ascidian Ciona intestinalis.Nucleic acids research · 2006Article
- Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.RNA (New York, N.Y.) · 2004Article
- Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.Molecular and cellular biology · 2004Article
- A global analysis of Caenorhabditis elegans operons.Nature · 2002Article
- abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis elegans.The Biochemical journal · 2002Article
- Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans.Molecular cell biology research communications : MCBRC · 2001Article
- Interplay between AAUAAA and the trans-splice site in processing of a Caenorhabditis elegans operon pre-mRNA.RNA (New York, N.Y.) · 2001Article
- Signals for pre-mRNA cleavage and polyadenylation.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Many Caenorhabditis elegans genes exist in operons in which polycistronic precursors are processed by cleavage at the 3' ends of upstream genes and trans splicing 100 to 400 nucleotides away, at the 5' ends of downstream genes, to generate monocistronic messages. Of the two spliced leaders, SL1 is trans spliced to the 5' ends of upstream genes, whereas SL2 is reserved for downstream genes in operons. However, there are isolated examples of what appears to be a different sort of operon, in which trans splicing is exclusively to SL1 and there is no intercistronic region; the polyadenylation signal is only a few base pairs upstream of the trans-splice site. We have analyzed the processing of an operon of this type by inserting the central part of mes-6/cks-1 into an SL2-type operon. In this novel context, cks-1 is trans spliced only to SL1, and mes-6 3'-end formation occurs normally, demonstrating that this unique mode of processing is indeed intrinsic to this kind of operon, which we herein designate "SL1-type." An exceptionally long polypyrimidine tract found in the 3' untranslated regions of the three known SL1-type operons is shown to be required for the accumulation of both upstream and downstream mRNAs. Mutations of the trans-splice and poly(A) signals indicate that the two processes are independent and in competition, presumably due to their close proximity, raising the possibility that production of upstream and downstream mRNAs is mutually exclusive.
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