ArticleMolecular and cellular biology1999
Evidence that protein binding specifies sites of DNA demethylation.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Biochemical and Epigenetic Regulation of Glutamate Metabolism in Maize (Plants (Basel, Switzerland) · 2024Article
- Epigenetic and epitranscriptomic regulation during oncogenicFrontiers in microbiology · 2024Review
- EBV-associated diseases: Current therapeutics and emerging technologies.Frontiers in immunology · 2022Review
- Epstein-Barr virus: a master epigenetic manipulator.Current opinion in virology · 2017Review
- The Role of Gammaherpesviruses in Cancer Pathogenesis.Pathogens (Basel, Switzerland) · 2016Review
- GC skew is a conserved property of unmethylated CpG island promoters across vertebrates.Nucleic acids research · 2015Article
- Conserved and divergent patterns of DNA methylation in higher vertebrates.Genome biology and evolution · 2014Article
- Local depletion of DNA methylation identifies a repressive p53 regulatory region in the NEK2 promoter.The Journal of biological chemistry · 2013Article
- TETonic shift: biological roles of TET proteins in DNA demethylation and transcription.Nature reviews. Molecular cell biology · 2013Review
- Epigenetic regulation of EBV and KSHV latency.Current opinion in virology · 2013Review
- Similarities between the Epstein-Barr Virus (EBV) Nuclear Protein EBNA1 and the Pioneer Transcription Factor FoxA: Is EBNA1 a "Bookmarking" Oncoprotein that Alters the Host Cell Epigenotype?Pathogens (Basel, Switzerland) · 2012Review
- Differential transcription factor use by the KIR2DL4 promoter under constitutive and IL-2/15-treated conditions.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- The importance of epigenetic alterations in the development of epstein-barr virus-related lymphomas.Mediterranean journal of hematology and infectious diseases · 2009Article
- Association of differential and site-dependent CpG methylation and diverse expression of DNA methyltransferases with the tissue-specific expression of human beta-globin gene in transgenic mice.International journal of hematology · 2009Article
- Chromatin profiling of Epstein-Barr virus latency control region.Journal of virology · 2007Article
- Active cytosine demethylation triggered by a nuclear receptor involves DNA strand breaks.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Regulation of Epstein-Barr virus latency type by the chromatin boundary factor CTCF.Journal of virology · 2006Article
- Article
- Site-selective in vivo targeting of cytosine-5 DNA methylation by zinc-finger proteins.Nucleic acids research · 2003Article
- The SDF1-G801A polymorphism is not associated with SDF1 gene expression in Epstein-Barr virus-transformed lymphoblastoid cells.Genes and immunity · 2003Article
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1 author.
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Abstract
It has been hypothesized that protein factors may protect CpG islands from methyltransferase during development and that demethylation may involve protein-DNA interactions at demethylated sites. However, direct evidence has been lacking. In this study, demethylation at the EBNA-1 binding sites of the Epstein-Barr virus latent replication origin, oriP, was investigated by using human cells. Several novel findings are discussed. First, there are specific preferential demethylation sites within the oriP region. Second, the DNA sequence of oriP alone is not the target of an active demethylation process. Third, EBNA-1 binding is required for the site-specific demethylation in oriP. Interestingly, CpG sites adjacent to and between the EBNA-1 sites do not become demethylated. Fourth, demethylation of the first DNA strand in oriP at the EBNA-1 binding sites involves a passive (replication-dependent) mechanism. The second-strand demethylation appears to occur through an active mechanism. That is, EBNA-1 protein binding prevents the EBNA-1 binding sites from being remethylated after one round of DNA replication, and it appears that an active demethylase then demethylates these hemimethylated sites. This study provides clear evidence that protein binding specifies sites of DNA demethylation and provides insights into the sequence of steps and the mechanism of demethylation.
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