ArticleThe EMBO journal1998
Cdc6 protein causes premature entry into S phase in a mammalian cell-free system.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 146 citations in OpenAlex.
- Computational Insights into SIRT1: Elucidating Mutational Impact on SIRT1-RECQL4 Structural Dynamics.Cell biochemistry and biophysics · 2026Article
- Stage-specific MCM protein expression inFrontiers in cellular and infection microbiology · 2025Article
- Cell cycle exits and U-turns: Quiescence as multiple reversible forms of arrest.Faculty reviews · 2023Review
- A G1-like state allows HIV-1 to bypass SAMHD1 restriction in macrophages.The EMBO journal · 2017Article
- Guttiferone K impedes cell cycle re-entry of quiescent prostate cancer cells via stabilization of FBXW7 and subsequent c-MYC degradation.Cell death & disease · 2016Article
- Simultaneous measurement of passage through the restriction point and MCM loading in single cells.Nucleic acids research · 2015Article
- Targeting of cytosolic phospholipase A2α impedes cell cycle re-entry of quiescent prostate cancer cells.Oncotarget · 2015Article
- Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication.Cell cycle (Georgetown, Tex.) · 2015Article
- In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization.Nucleic acids research · 2013Article
- Relationship between DNA replication and the nuclear matrix.Genes to cells : devoted to molecular & cellular mechanisms · 2013Review
- Hypoxic activation of ATR and the suppression of the initiation of DNA replication through cdc6 degradation.Oncogene · 2012Article
- Meier-Gorlin syndrome mutations disrupt an Orc1 CDK inhibitory domain and cause centrosome reduplication.Genes & development · 2012Article
- Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro.Journal of cell science · 2010Article
- DNA replication licensing factors and aneuploidy are linked to tumor cell cycle state and clinical outcome in penile carcinoma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2009Article
- HOXD13 binds DNA replication origins to promote origin licensing and is inhibited by geminin.Molecular and cellular biology · 2009Article
- Reduced expression of GINS complex members induces hallmarks of pre-malignancy in primary untransformed human cells.Cell cycle (Georgetown, Tex.) · 2009Article
- Cell-cycle-phase progression analysis identifies unique phenotypes of major prognostic and predictive significance in breast cancer.British journal of cancer · 2009Article
- Article
- Identification of an arginine-rich motif in human papillomavirus type 1 E1;E4 protein necessary for E4-mediated inhibition of cellular DNA synthesis in vitro and in cells.Journal of virology · 2008Article
- Diagnosis of pancreaticobiliary malignancy by detection of minichromosome maintenance protein 5 in bile aspirates.British journal of cancer · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We exploit an improved mammalian cell-free DNA replication system to analyse quiescence and Cdc6 function. Quiescent 3T3 nuclei cannot initiate replication in S phase cytosol from HeLa or 3T3 cells. Following release from quiescence, nuclei become competent to initiate semiconservative DNA replication in S phase cytosol, but not in G0 phase cytosol. Immunoblots show that quiescent cells lack Cdc6 and that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum Cdc6 accumulation and MCM protein binding to chromatin in vivo. Addition of recombinant Cdc6 to permeabilized, but not intact, G1 nuclei causes up to 82% of the nuclei to initiate and accelerates G1 progression, making nuclei competent to replicate prematurely.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.