Evidence map›Paper›PMID 9854071›Full record

ArticleJournal of clinical microbiology1999

Rapid identification of clinically relevant Nocardia species to genus level by 16S rRNA gene PCR.

F J Laurent, F Provost, P Boiron

Erratum issuedOpen access · bronzeAbstract readComparative Study
In one paragraph

Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
6.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 140 citations in OpenAlex.

  1. Pathogens (Basel, Switzerland) · 2026
    Article
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  5. Microbial risk assessment ofComputational and structural biotechnology journal · 2021
    Article
  6. Article
  7. Article
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  9. Detection ofEthiopian journal of health sciences · 2019
    Article
  10. Article
  11. Surgical neurology international · 2019
    Article
  12. Article
  13. Successful Treatment of CutaneousFrontiers in veterinary science · 2019
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. The Surgical Treatment of Mycetoma.PLoS neglected tropical diseases · 2016
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

F J LaurentInstitut Pasteur, Unite de Mycologie, Centre National de Reference des Mycoses Humaines, des Antifongiques et des Actinomycetes, 75724 Paris Cedex 15, France. laurent@lyon-sud.univ-lyon1.fr
F Provost
P Boiron
Institut Pasteur · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two regions of the gene coding for 16S rRNA in Nocardia species were selected as genus-specific primer sequences for a PCR assay. The PCR protocol was tested with 60 strains of clinically relevant Nocardia isolates and type strains. It gave positive results for all strains tested. Conversely, the PCR assay was negative for all tested species belonging to the most closely related genera, including Dietzia, Gordona, Mycobacterium, Rhodococcus, Streptomyces, and Tsukamurella. Besides, unlike the latter group of isolates, all Nocardia strains exhibited one MlnI recognition site but no SacI restriction site. This assay offers a specific and rapid alternative to chemotaxonomic methods for the identification of Nocardia spp. isolated from pathogenic samples.

Indexed as

Bacteriological TechniquesBase SequenceHumansMolecular Sequence DataNocardiaPolymerase Chain ReactionRNA, BacterialRNA, Ribosomal, 16SSensitivity and SpecificitySequence AlignmentSpecies SpecificityRNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID9854071
PMCPMC84177
OpenAlexW1513924779

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.