ArticleJournal of clinical microbiology1999
Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 273 citations in OpenAlex.
- Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence In situ hybridization probes.Journal of clinical microbiology · 2000Trial
- Bibliometric Review on New Possibilities of Antimycobacterial Agents: Exploring Siderophore Desferrioxamine's Applications as an Antimicrobial Agent.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of MajorJournal of clinical microbiology · 2020Article
- Mycobacterial identification on homogenised biopsy facilitates the early diagnosis and treatment of laryngeal tuberculosis.Open medicine (Warsaw, Poland) · 2020Article
- Mycobacterial species and their contribution to cholesterol degradation in wastewater treatment plants.Scientific reports · 2019Article
- Current Affairs of Microbial Genome-Wide Association Studies: Approaches, Bottlenecks and Analytical Pitfalls.Frontiers in microbiology · 2019Review
- Genotyping Multidrug-Resistant Mycobacterium tuberculosis from Primary Sputum and Decontaminated Sediment with an Integrated Microfluidic Amplification Microarray Test.Journal of clinical microbiology · 2018Article
- Rapid Detection of Streptomycin-ResistantAdvanced biomedical research · 2017Article
- Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.Journal of clinical microbiology · 2015Article
- Simplified microarray system for simultaneously detecting rifampin, isoniazid, ethambutol, and streptomycin resistance markers in Mycobacterium tuberculosis.Journal of clinical microbiology · 2014Article
- Demonstrating a multi-drug resistant Mycobacterium tuberculosis amplification microarray.Journal of visualized experiments : JoVE · 2014Article
- A differential fluorescent receptor for nucleic acid analysis.Chembiochem : a European journal of chemical biology · 2014Article
- Visual detection of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis strains by use of an asymmetrically split peroxidase DNAzyme.Journal of clinical microbiology · 2012Article
- Capillary electrophoresis-single strand conformation polymorphism for the detection of multiple mutations leading to tuberculosis drug resistance.Journal of microbiological methods · 2012Article
- Molecular logic gates for DNA analysis: detection of rifampin resistance in M. tuberculosis DNA.Angewandte Chemie (International ed. in English) · 2012Article
- Infections in Heart and Lung Transplant Recipients.Clinical microbiology newsletter · 2012Article
- Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.Clinical microbiology reviews · 2011Review
- Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.Journal of clinical microbiology · 2010Article
- Future Veterinary Diagnostics.Journal of exotic pet medicine · 2010Article
- Detection of HIV cDNA point mutations with rolling-circle amplification arrays.Molecules (Basel, Switzerland) · 2010Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species identification within the genus Mycobacterium and subsequent antibiotic susceptibility testing still rely on time-consuming, culture-based methods. Despite the recent development of DNA probes, which greatly reduce assay time, there is a need for a single platform assay capable of answering the multitude of diagnostic questions associated with this genus. We describe the use of a DNA probe array based on two sequence databases: one for the species identification of mycobacteria (82 unique 16S rRNA sequences corresponding to 54 phenotypical species) and the other for detecting Mycobacterium tuberculosis rifampin resistance (rpoB alleles). Species identification or rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacterial colonies to the array. Seventy mycobacterial isolates from 27 different species and 15 rifampin-resistant M. tuberculosis strains were tested. A total of 26 of 27 species were correctly identified as well as all of the rpoB mutants. This parallel testing format opens new perspectives in terms of patient management for bacterial diseases by allowing a number of genetic tests to be simultaneously run.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.