ArticleThe Biochemical journal1999
Age-related changes in the synthesis of link protein and aggrecan in human articular cartilage: implications for aggregate stability.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 67 citations in OpenAlex.
- Bidirectional adverse effects of low and high atmospheric humidity on arthritis in middle-aged and older adults: evidence from propensity score matching and comprehensive mosdeling approaches.BMC public health · 2026Article
- Biochemical Aspects of Scaffolds for Cartilage Tissue Engineering; from Basic Science to Regenerative Medicine.The archives of bone and joint surgery · 2022Review
- Serum cartilage oligomeric matrix protein as a biomarker for predicting development and progression of knee osteoarthritis.International orthopaedics · 2021Article
- Regulatory mechanisms and clinical manifestations of musculoskeletal aging.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Review
- Combating Osteoarthritis through Stem Cell Therapies by Rejuvenating Cartilage: A Review.Stem cells international · 2018Review
- Articular cartilage changes in maturing athletes: new targets for joint rejuvenation.Sports health · 2014Article
- The age-related changes in cartilage and osteoarthritis.BioMed research international · 2013Review
- Events in articular chondrocytes with aging.Current osteoporosis reports · 2011Review
- Ultrasonic probe is useful for in vivo quantitative assessment of medial femoral condyle articular cartilage.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2011Article
- Article
- Adult equine bone marrow stromal cells produce a cartilage-like ECM mechanically superior to animal-matched adult chondrocytes.Matrix biology : journal of the International Society for Matrix Biology · 2010Article
- Dynamic mechanical loading enhances functional properties of tissue-engineered cartilage using mature canine chondrocytes.Tissue engineering. Part A · 2010Article
- Effects of dexamethasone on the functional properties of cartilage explants during long-term culture.The American journal of sports medicine · 2010Article
- A comparison of primary and passaged chondrocytes for use in engineering the temporomandibular joint.Archives of oral biology · 2009Article
- Diminished mitochondrial DNA integrity and repair capacity in OA chondrocytes.Osteoarthritis and cartilage · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rates of incorporation of radiolabelled leucine into aggrecan and link protein have been measured in human articular cartilage of different ages. Aggrecan and link protein were purified in the A1 fraction of CsCl gradients as a result of their ability to form high-buoyant-density proteoglycan aggregates with hyaluronic acid. Separation of the aggrecan from the link protein was achieved by Mono Q anion-exchange chromatography. The rates of synthesis of both aggrecan and link protein decreased with age. The age-related decrease in synthesis of aggrecan was paralleled by a decrease in the rate of sulphate incorporation into glycosaminoglycan chains. The synthesis of link protein decreased with age to a greater extent than that of aggrecan such that the ratio of the rates of link protein to aggrecan synthesis decreased from 1 in immature cartilage to 0.2 in mature cartilage. The age-related decrease in link protein synthesis is controlled at least in part by transcriptional or post-transcriptional mechanisms, as shown by the accompanying age-related decrease in link-protein mRNA. The absence of any age-related decrease in aggrecan mRNA suggests that the decrease in synthesis of aggrecan core protein is controlled by a translational mechanism. Measurement of the total tissue content of aggrecan and link protein by radioimmunoassay revealed an age-related increase in the accumulation of these matrix proteins, even though their de novo synthesis was decreasing. This illustrates the importance that the regulation of extracellular post-translational modification also has in controlling the overall turnover of the cartilage matrix.
Indexed as
Identifiers
9854027PMC1219938W2108740080What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.