ArticleThe Journal of physiology1999
Interference of H2O2 with stimulus-secretion coupling in mouse pancreatic beta-cells.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 107 citations in OpenAlex.
- Article
- Rpl13a small nucleolar RNAs regulate systemic glucose metabolism.The Journal of clinical investigation · 2016Article
- Survival of encapsulated islets: More than a membrane story.World journal of transplantation · 2016Review
- Cystine accumulation attenuates insulin release from the pancreatic β-cell due to elevated oxidative stress and decreased ATP levels.The Journal of physiology · 2015Article
- A change of heart: oxidative stress in governing muscle function?Biophysical reviews · 2015Article
- Inhibitory and excitatory neuromodulation by hydrogen peroxide: translating energetics to information.The Journal of physiology · 2015Review
- Hypoxia-dependent reactive oxygen species signaling in the pulmonary circulation: focus on ion channels.Antioxidants & redox signaling · 2015Review
- Article
- Postranslational Modification of Ion Channels in Colonic Inflammation.Current neuropharmacology · 2015Review
- Palmitate induces reactive oxygen species production and β-cell dysfunction by activating nicotinamide adenine dinucleotide phosphate oxidase through Src signaling.Journal of diabetes investigation · 2014Article
- Cooked common beans (Phaseolus vulgaris) protect against β-cell damage in streptozotocin-induced diabetic rats.Plant foods for human nutrition (Dordrecht, Netherlands) · 2013Article
- Article
- Does NAD(P)H oxidase-derived H2O2 participate in hypotonicity-induced insulin release by activating VRAC in β-cells?Pflugers Archiv : European journal of physiology · 2012Article
- The level of menadione redox-cycling in pancreatic β-cells is proportional to the glucose concentration: role of NADH and consequences for insulin secretion.Toxicology and applied pharmacology · 2012Article
- Expression of transient receptor potential ankyrin 1 (TRPA1) and its role in insulin release from rat pancreatic beta cells.PloS one · 2012Article
- Subsecond regulation of striatal dopamine release by pre-synaptic KATP channels.Journal of neurochemistry · 2011Article
- H2O2: a dynamic neuromodulator.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2011Review
- Article
- Molecular basis and structural insight of vascular K(ATP) channel gating by S-glutathionylation.The Journal of biological chemistry · 2011Article
- BK channels affect glucose homeostasis and cell viability of murine pancreatic beta cells.Diabetologia · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have reported previously that in mouse pancreatic beta-cells H2O2 hyperpolarizes the membrane and increases the ATP-sensitive K+ current recorded in the perforated patch configuration of the patch-clamp technique. The present study was undertaken to elucidate the underlying mechanisms. 2. The intracellular ATP concentration measured by chemoluminescence was reduced by H2O2. The ADP concentration increased in parallel during the first 10 min, resulting in a pronounced decrease in the ATP/ADP ratio. 3. Consistent with these results, glucose-stimulated insulin secretion from isolated islets was inhibited by H2O2. 4. Membrane hyperpolarization measured with intracellular microelectrodes in intact islets and inhibition of insulin secretion were counteracted by tolbutamide, indicating that the channels are still responsive to inhibitors and that the ATP concentration is not too low to trigger exocytosis. However, the sensitivity of the beta-cells to tolbutamide was reduced after treatment with H2O2. 5. H2O2 increased the intracellular Ca2+ activity ([Ca2+]i) in a biphasic manner. A first transient rise in [Ca2+]i due to mobilization of Ca2+ from intracellular stores was followed by a sustained increase, which was at least partly dependent on Ca2+ influx. The first phase seems to reflect Ca2+ mobilization from mitochondria. 6. Our results demonstrate that H2O2 interferes with glucose metabolism, which influences the membrane potential and ATP-sensitive K+ current via the intracellular concentration of ATP. These events finally lead to an inhibition of insulin secretion despite an increase in [Ca2+]i.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.