ArticleThe Journal of cell biology1998
The localization of myosin VI at the golgi complex and leading edge of fibroblasts and its phosphorylation and recruitment into membrane ruffles of A431 cells after growth factor stimulation.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
81 citing papers in PubMed, 197 citations in OpenAlex.
- Myosin VI is involved in the structural organization and molecular composition of epididymal epithelial cells in mouse†.Biology of reproduction · 2026Article
- Antenatal phenotype associated with PAK2 pathogenic variants: bilateral pleural effusion as a warning sign.BMC medical genomics · 2025Article
- Post-translational modifications of vertebrate striated muscle myosin heavy chains.Cytoskeleton (Hoboken, N.J.) · 2024Review
- The actomyosin system is essential for the integrity of the endosomal system in bloodstream formeLife · 2024Article
- Motor domain phosphorylation increases nucleotide exchange and turns MYO6 into a faster and stronger motor.Nature communications · 2024Article
- Autoinhibition and activation of myosin VI revealed by its cryo-EM structure.Nature communications · 2024Article
- The twoFrontiers in physiology · 2024Article
- The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia.Developmental cell · 2023Article
- Loss of Unconventional Myosin VI Affects cAMP/PKA Signaling in Hindlimb Skeletal Muscle in an Age-Dependent Manner.Frontiers in physiology · 2022Article
- New methods to decrypt emerging macropinosome functions during the host-pathogen crosstalk.Cellular microbiology · 2021Review
- Diverse functions of myosin VI in spermiogenesis.Histochemistry and cell biology · 2021Review
- The cross-talk of energy sensing and mitochondrial anchoring sustains synaptic efficacy by maintaining presynaptic metabolism.Nature metabolism · 2020Article
- Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis†.Biology of reproduction · 2020Article
- OPTN recruitment to a Golgi-proximal compartment regulates immune signalling and cytokine secretion.Journal of cell science · 2020Article
- Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis†‡.Biology of reproduction · 2020Article
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics.EMBO reports · 2018Article
- Myosin VI-Dependent Actin Cages Encapsulate Parkin-Positive Damaged Mitochondria.Developmental cell · 2018Article
- Optineurin: A Coordinator of Membrane-Associated Cargo Trafficking and Autophagy.Frontiers in immunology · 2018Review
- Expression and localization of myosin VI in developing mouse spermatids.Histochemistry and cell biology · 2017Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myosin VI is an unconventional myosin that may play a role in vesicular membrane traffic through actin rich regions of the cytoplasm in eukaryotic cells. In this study we have cloned and sequenced a cDNA encoding a chicken intestinal brush border myosin VI. Polyclonal antisera were raised to bacterially expressed fragments of this myosin VI. The affinity purified antibodies were highly specific for myosin VI by immunoblotting and immunoprecipitation and were used to study the localization of the protein by immunofluorescence and immunoelectron microscopy. It was found that in NRK and A431 cells, myosin VI was associated with both the Golgi complex and the leading, ruffling edge of the cell as well as being present in a cytosolic pool. In A431 cells in which cell surface ruffling was stimulated by EGF, myosin VI was phosphorylated and recruited into the newly formed ruffles along with ezrin and myosin V. In vitro experiments suggested that a p21-activated kinase (PAK) might be the kinase responsible for phosphorylation in the motor domain. These results strongly support a role for myosin VI in membrane traffic on secretory and endocytic pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.