ArticleJournal of bacteriology1998
Comparison in vitro of a high- and a low-abundance chemoreceptor of Escherichia coli: similar kinase activation but different methyl-accepting activities.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- Methyltransferase CheR binds to its chemoreceptor substrates independent of their signaling conformation yet modifies them differentially.Protein science : a publication of the Protein Society · 2020Article
- Blue Light Is a Universal Signal forJournal of bacteriology · 2019Article
- Signaling complexes control the chemotaxis kinase by altering its apparent rate constant of autophosphorylation.Protein science : a publication of the Protein Society · 2017Article
- Bacterial Energy Sensor Aer Modulates the Activity of the Chemotaxis Kinase CheA Based on the Redox State of the Flavin Cofactor.The Journal of biological chemistry · 2016Article
- Engineered bacteria detect spatial profiles in glucose concentration within solid tumor cell masses.Biotechnology and bioengineering · 2016Article
- Universal response-adaptation relation in bacterial chemotaxis.Journal of bacteriology · 2015Article
- Selective allosteric coupling in core chemotaxis signaling complexes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Influence of membrane lipid composition on a transmembrane bacterial chemoreceptor.The Journal of biological chemistry · 2012Article
- Direct evidence that the carboxyl-terminal sequence of a bacterial chemoreceptor is an unstructured linker and enzyme tether.Protein science : a publication of the Protein Society · 2011Article
- Core unit of chemotaxis signaling complexes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Chemotaxis kinase CheA is activated by three neighbouring chemoreceptor dimers as effectively as by receptor clusters.Molecular microbiology · 2011Article
- Chemoreceptors in signalling complexes: shifted conformation and asymmetric coupling.Molecular microbiology · 2010Article
- The chemoreceptor dimer is the unit of conformational coupling and transmembrane signaling.Journal of bacteriology · 2010Article
- Characterization of the Thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase CheR:MCP tethering.Molecular microbiology · 2007Article
- Nanodiscs separate chemoreceptor oligomeric states and reveal their signaling properties.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Similarities and differences in interactions of the activity-enhancing chemoreceptor pentapeptide with the two enzymes of adaptational modification.Journal of bacteriology · 2006Article
- Carboxyl-terminal extensions beyond the conserved pentapeptide reduce rates of chemoreceptor adaptational modification.Journal of bacteriology · 2005Article
- Site-specific and synergistic stimulation of methylation on the bacterial chemotaxis receptor Tsr by serine and CheW.BMC microbiology · 2005Article
- Ligand-specific activation of Escherichia coli chemoreceptor transmethylation.Journal of bacteriology · 2004Article
- Diversity in chemotaxis mechanisms among the bacteria and archaea.Microbiology and molecular biology reviews : MMBR · 2004Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Escherichia coli, high-abundance chemoreceptors are present in cellular amounts approximately 10-fold greater than low-abundance chemoreceptors. Cells containing only low-abundance receptors exhibit abnormally low tumble frequencies and do not migrate effectively in spatial gradients. These defects reflect an inherent activity difference between the two receptor classes. We used in vitro assays to investigate this difference. The low-abundance receptor Trg mediated an approximately 100-fold activation of the kinase CheA, only twofold less than activation by the high-abundance receptor Tar. In contrast, Trg was less than 1/20 as active as Tar for in vitro methylation. As observed for high-abundance receptors, kinase activation by Trg varied with the extend of modification at methyl-accepting sites; low methylation corresponded to low kinase activation. Thus, in Trg-only cells, low receptor methylation would result in low kinase activation, correspondingly low content of phospho-CheY, and a decreased dynamic range over which attractant binding could modulate kinase activity. These features could account for the low tumble frequency and inefficient taxis exhibited by Trg-only cells. Thus, the crucial functional difference between the receptor classes is likely to be methyl-accepting activity. We investigated the structural basis for this functional difference by introducing onto the carboxy terminus of Trg a CheR-binding pentapeptide, usually found only at the carboxy termini of high-abundance receptors. This addition enhanced the in vitro methyl-accepting activity of Trg 10-fold.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.