ArticleJournal of bacteriology1998
Physiological role for the GlnK protein of enteric bacteria: relief of NifL inhibition under nitrogen-limiting conditions.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 100 citations in OpenAlex.
- Nitrogenase regulation inbioRxiv : the preprint server for biology · 2026Article
- Molecular Origins of Transcriptional Heterogeneity in Diazotrophic Klebsiella oxytoca.mSystems · 2022Article
- The Oxoglutarate Binding Site and Regulatory Mechanism Are Conserved in Ammonium Transporter Inhibitors GlnKs fromInternational journal of molecular sciences · 2021Article
- Resource Allocation During the Transition to Diazotrophy inFrontiers in microbiology · 2021Article
- Manipulating nitrogen regulation in diazotrophic bacteria for agronomic benefit.Biochemical Society transactions · 2019Review
- The pivotal twin histidines and aromatic triad of the Escherichia coli ammonium channel AmtB can be replaced.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Insights into membrane association of Klebsiella pneumoniae NifL under nitrogen-fixing conditions from mutational analysis.Journal of bacteriology · 2011Article
- Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1.BMC microbiology · 2011Article
- Genetic evidence for an essential oscillation of transmembrane-spanning segment 5 in the Escherichia coli ammonium channel AmtB.Genetics · 2009Article
- Effect of perturbation of ATP level on the activity and regulation of nitrogenase in Rhodospirillum rubrum.Journal of bacteriology · 2009Article
- Identification and functional characterization of NifA variants that are independent of GlnB activation in the photosynthetic bacterium Rhodospirillum rubrum.Microbiology (Reading, England) · 2008Article
- Membrane sequestration of PII proteins and nitrogenase regulation in the photosynthetic bacterium Rhodobacter capsulatus.Journal of bacteriology · 2007Article
- Functional analysis of the GAF domain of NifA in Azospirillum brasilense: effects of Tyr-->Phe mutations on NifA and its interaction with GlnB.Molecular genetics and genomics : MGG · 2005Article
- GlnD is essential for NifA activation, NtrB/NtrC-regulated gene expression, and posttranslational regulation of nitrogenase activity in the photosynthetic, nitrogen-fixing bacterium Rhodospirillum rubrum.Journal of bacteriology · 2005Article
- Identification of critical residues in GlnB for its activation of NifA activity in the photosynthetic bacterium Rhodospirillum rubrum.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The NifL-NifA System: a multidomain transcriptional regulatory complex that integrates environmental signals.Journal of bacteriology · 2004Review
- Physiological studies of Escherichia coli strain MG1655: growth defects and apparent cross-regulation of gene expression.Journal of bacteriology · 2003Article
- Signal transduction protein P(II) is required for NtcA-regulated gene expression during nitrogen deprivation in the cyanobacterium Synechococcus elongatus strain PCC 7942.Journal of bacteriology · 2003Article
- Fnr is involved in oxygen control of Herbaspirillum seropedicae N-truncated NifA protein activity in Escherichia coli.Applied and environmental microbiology · 2003Article
- Context-dependent functions of the PII and GlnK signal transduction proteins in Escherichia coli.Journal of bacteriology · 2002Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Klebsiella pneumoniae, NifA-dependent transcription of nitrogen fixation (nif) genes is inhibited by a flavoprotein, NifL, in the presence of molecular oxygen and/or combined nitrogen. We recently demonstrated that the general nitrogen regulator NtrC is required to relieve NifL inhibition under nitrogen (N)-limiting conditions. We provide evidence that the sole basis for the NtrC requirement is its role as an activator of transcription for glnK, which encodes a PII-like allosteric effector. Relief of NifL inhibition is a unique physiological function for GlnK in that the structurally related GlnB protein of enteric bacteria-apparently a paralogue of GlnK-cannot substitute. Unexpectedly, although covalent modification of GlnK by uridylylation normally occurs under N-limiting conditions, several lines of evidence indicate that uridylylation is not required for relief of NifL inhibition. When GlnK was synthesized constitutively from non-NtrC-dependent promoters, it was able to relieve NifL inhibition in the absence of uridylyltransferase, the product of the glnD gene, and under N excess conditions. Moreover, an altered form of GlnK, GlnKY51N, which cannot be uridylylated due to the absence of the requisite tyrosine, was still able to relieve NifL inhibition.
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