ArticleJournal of bacteriology1998
Stability of the Agrobacterium tumefaciens VirB10 protein is modulated by growth temperature and periplasmic osmoadaption.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.ACS synthetic biology · 2026Article
- A Proposal for a Consolidated Structural Model of the CagY Protein ofInternational journal of molecular sciences · 2023Article
- Multiple Mechanisms Drive the Evolutionary Adaptation ofJournal of fungi (Basel, Switzerland) · 2021Article
- Effector Avr4 inFrontiers in microbiology · 2021Article
- Evidence for intragenic recombination and selective sweep in an effector gene ofEvolutionary applications · 2018Article
- The EnvZ-OmpR Two-Component Signaling System Is Inactivated in a Mutant Devoid of Osmoregulated Periplasmic Glucans inFrontiers in microbiology · 2018Article
- Osmoregulated Periplasmic Glucans.EcoSal Plus · 2017Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Cold adaptation regulated by cryptic prophage excision in Shewanella oneidensis.The ISME journal · 2016Article
- Diverse mechanisms shape the evolution of virulence factors in the potato late blight pathogen Phytophthora infestans sampled from China.Scientific reports · 2016Article
- Evaluation of the Role of the opgGH Operon in Yersinia pseudotuberculosis and Its Deletion during the Emergence of Yersinia pestis.Infection and immunity · 2015Article
- Unmasking host and microbial strategies in the Agrobacterium-plant defense tango.Frontiers in plant science · 2015Review
- Review
- Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant.FEBS open bio · 2012Article
- Study of free oligosaccharides derived from the bacterial N-glycosylation pathway.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Microbial thermosensors.Cellular and molecular life sciences : CMLS · 2009Review
- Replicon-specific regulation of small heat shock genes in Agrobacterium tumefaciens.Journal of bacteriology · 2004Article
- Molecular cloning and characterization of cgt, the Brucella abortus cyclic beta-1,2-glucan transporter gene, and its role in virulence.Infection and immunity · 2004Article
- A novel cytology-based, two-hybrid screen for bacteria applied to protein-protein interaction studies of a type IV secretion system.Journal of bacteriology · 2002Article
- Elevated temperature differentially affects virulence, VirB protein accumulation, and T-pilus formation in different Agrobacterium tumefaciens and Agrobacterium vitis strains.Journal of bacteriology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Export of oncogenic T-DNA from the phytopathogen Agrobacterium tumefaciens is mediated by the products of the virB operon. It has recently been reported (K. J. Fullner and E. W. Nester, J. Bacteriol. 178:1498-1504, 1996) that DNA transfer does not occur at elevated temperatures; these observations correlate well with much earlier studies on the temperature sensitivity of crown gall tumor development on plants. In testing the hypothesis that this loss of DNA movement reflects a defect in assembly or maintenance of a stable DNA transfer machinery at high temperature, we have found that steady-state levels of VirB10 are sensitive to growth temperature while levels of several other VirB proteins are considerably less affected. This temperature-dependent failure to accumulate VirB10 is exacerbated in an attachment-deficient mutant strain (chvB) which exhibits pleiotropic defects in periplasmic osmoadaption, and virulence of a chvB mutant can be partially restored by lowering the temperature at which the bacteria and the plant tissue are cocultivated. Furthermore, the stability of VirB10 is diminished in cells lacking functional VirB9, but only under conditions of low osmolarity. We propose that newly synthesized VirB10 is inherently labile in the presence of a large osmotic gradient across the inner membrane and is rapidly degraded unless it is stabilized by VirB9-dependent assembly into oligomeric complexes. The possibility that VirB10-containing complexes are not assembled properly at elevated temperatures suggests an explanation for the decades-old observation that tumor formation is exquisitely sensitive to ambient temperature.
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