ArticleRNA (New York, N.Y.)1998
Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 81 citations in OpenAlex.
- Ecd promotes U5 snRNP maturation and Prp8 stability.Nucleic acids research · 2021Article
- Genetics and biochemistry remain essential in the structural era of the spliceosome.Methods (San Diego, Calif.) · 2017Review
- Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength.Genome biology · 2015Article
- Article
- Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection.Nucleic acids research · 2015Article
- Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.Eukaryotic cell · 2013Article
- RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.The EMBO journal · 2013Article
- A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing.Molecular and cellular biology · 2013Article
- Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly.Nucleic acids research · 2013Article
- The recruitment of the U5 snRNP to nascent transcripts requires internal loop 1 of U5 snRNA.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Splint ligation of RNA with T4 DNA ligase.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome.RNA (New York, N.Y.) · 2009Article
- Computational identification of four spliceosomal snRNAs from the deep-branching eukaryote Giardia intestinalis.PloS one · 2008Article
- A dynamic bulge in the U6 RNA internal stem-loop functions in spliceosome assembly and activation.RNA (New York, N.Y.) · 2007Article
- Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.RNA (New York, N.Y.) · 2007Article
- The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP.RNA (New York, N.Y.) · 2006Article
- Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome.RNA (New York, N.Y.) · 2006Article
- Alternative splicing regulation at tandem 3' splice sites.Nucleic acids research · 2006Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present here the first insights into the organization of proteins on the RNA in the U5 snRNP of Saccharomyces cerevisiae. Photo-crosslinking with uniformly labeled U5 RNA in snRNPs reconstituted in vitro revealed five contacting proteins, Prp8p, Snu114p, p30, p16, and p10, contact by the three smaller proteins requiring an intact Sm site. Site-specific crosslinking showed that Snu114p contacts the 5' side of internal loop 1, whereas Prp8p interacts with five different regions of the 5' stem-loop, but not with the Sm site or 3' stem-loop. Both internal loops in the 5' domain are essential for Prp8p to associate with the snRNP, but the conserved loop 1 is not, although this is the region to which Prp8p crosslinks most strongly. The extensive contacts between Prp8p and the 5' stem-loop of U5 RNA support the hypothesis that, in spliceosomes, Prp8p stabilizes loop 1-exon interactions. Moreover, data showing that Prp8p contacts the exons even in the absence of loop 1 indicate that Prp8p may be the principal anchoring factor for exons in the spliceosome. This and the close proximity of the spliceosomal translocase, Snu114p, to U5 loop 1 and Prp8p support and extend the proposal that Snu114p mimics U5 loop 1 during a translocation event in the spliceosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.