ArticleJournal of virology1999
Efficient transduction by an amphotropic retrovirus vector is dependent on high-level expression of the cell surface virus receptor.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 67 citations in OpenAlex.
- Carbohydrates: Binding Sites and Potential Drug Targets for Neural-Affecting Pathogens.Advances in neurobiology · 2023Article
- Xpr1 is an atypical G-protein-coupled receptor that mediates xenotropic and polytropic murine retrovirus neurotoxicity.Journal of virology · 2012Article
- A novel envelope mediated post entry restriction of murine leukaemia virus in human cells is Ref1/TRIM5α independent.Retrovirology · 2010Article
- Transduction of human primitive repopulating hematopoietic cells with lentiviral vectors pseudotyped with various envelope proteins.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Cocal-pseudotyped lentiviral vectors resist inactivation by human serum and efficiently transduce primate hematopoietic repopulating cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Ephrin-B2 expression critically influences Nipah virus infection independent of its cytoplasmic tail.Virology journal · 2008Article
- Feline leukemia virus T entry is dependent on both expression levels and specific interactions between cofactor and receptor.Virology · 2007Article
- Importance of receptor usage, Fli1 activation, and mouse strain for the stem cell specificity of 10A1 murine leukemia virus leukemogenicity.Journal of virology · 2007Article
- Use of different but overlapping determinants in a retrovirus receptor accounts for non-reciprocal interference between xenotropic and polytropic murine leukemia viruses.Retrovirology · 2005Article
- Article
- Expression and characterization of a soluble, active form of the jaagsiekte sheep retrovirus receptor, Hyal2.Journal of virology · 2005Article
- Cell targeting in anti-cancer gene therapy.The Malaysian journal of medical sciences : MJMS · 2004Article
- Determination of infectious retrovirus concentration from colony-forming assay with quantitative analysis.Journal of virology · 2003Article
- Role of virus receptor Hyal2 in oncogenic transformation of rodent fibroblasts by sheep betaretrovirus env proteins.Journal of virology · 2003Article
- G100R mutation within 4070A murine leukemia virus Env increases virus receptor binding, kinetics of entry, and viral transduction efficiency.Journal of virology · 2003Article
- Similar regulation of cell surface human T-cell leukemia virus type 1 (HTLV-1) surface binding proteins in cells highly and poorly transduced by HTLV-1-pseudotyped virions.Journal of virology · 2002Article
- Heparin binds to murine leukemia virus and inhibits Env-independent attachment and infection.Journal of virology · 2002Article
- Gene delivery into primary T cells: overview and characterization of a transgenic model for efficient adenoviral transduction.Immunologic research · 2002Review
- Envelope fusion protein binding studies in an inducible model of retrovirus receptor expression and in CD34(+) cells emphasize limited transduction at low receptor levels.Gene therapy · 2001Article
- Retroviral-mediated gene transfer in primary murine and human T-lymphocytes.Molecular biotechnology · 2000Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Transduction by murine leukemia virus-based retrovirus vectors is limited in certain cell types, particularly in nondividing cells. But transduction can be inefficient even in cells that divide rapidly. For example, exposure of 208F rat embryo fibroblasts to an excess of an amphotropic retrovirus vector encoding alkaline phosphatase results in a transduction efficiency of only about 10%, even though these cells divide rapidly. Here we show that transduction of 208F cells is limited by cell surface retrovirus receptor levels; overexpression of the amphotropic retrovirus receptor Pit2 markedly improved the transduction efficiency to 50%. To characterize receptor levels and binding affinity, we synthesized a fusion protein that joins the amino terminus of the amphotropic envelope protein to the Fc region of a human immunoglobulin G1 molecule for use in binding assays. In comparison to the parental cell line, the modified cell line showed an order of magnitude increase in binding sites of from 18,000 to 150,000 per cell. Thus, efficient transduction by an amphotropic retrovirus vector requires high-level expression of the retrovirus receptor Pit2. These results provide the rationale for further examination of the role of receptor levels in inefficient transduction, especially with regard to target cells for gene therapy, where a high transduction rate is often crucial.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.