ArticleProceedings of the National Academy of Sciences of the United States of America1998
Carbohydrate binding and resistance to proteolysis control insecticidal activity of Griffonia simplicifolia lectin II.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 123 citations in OpenAlex.
- Recombinant lectin from the coastal jack bean Canavalia rosea and its anti-bruchid activity towards Callosobruchus maculatus (Chrysomelidae: Coleoptera).Die Naturwissenschaften · 2026Article
- Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor.ACS sensors · 2023Article
- Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor.bioRxiv : the preprint server for biology · 2023Article
- Expression of Modified Snowdrop Lectin (Genes · 2022Article
- Evaluation of the Potential of a Lectin Extracted fromMolecules (Basel, Switzerland) · 2022Article
- Research advances and prospects of legume lectins.Journal of biosciences · 2021Review
- Transcriptomic analysis of differentially expressed genes in the oviduct of Rhacophorus omeimontis provides insights into foam nest construction.BMC genomics · 2019Article
- Chemical Characterization and DNA Fingerprinting ofMolecules (Basel, Switzerland) · 2019Article
- Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper).Scientific reports · 2019Article
- Rapid Drop-Test for Lectin Binding with Glycopolymer-Coated Optical Ring Resonators.Biosensors · 2019Article
- The Digestive System of the Two-Spotted Spider Mite,Frontiers in plant science · 2018Article
- Potential of the Lectin/Inhibitor Isolated from Crataeva tapia Bark (CrataBL) for Controlling Callosobruchus maculatus Larva Development.Journal of agricultural and food chemistry · 2015Article
- Effect of Myracrodruon urundeuva leaf lectin on survival and digestive enzymes of Aedes aegypti larvae.Parasitology research · 2012Article
- Foam nest components of the túngara frog: a cocktail of proteins conferring physical and biological resilience.Proceedings. Biological sciences · 2009Article
- Structure and function of Pseudomonas aeruginosa protein PA1324 (21-170).Protein science : a publication of the Protein Society · 2009Article
- Arthropod-inducible proteins: broad spectrum defenses against multiple herbivores.Plant physiology · 2008Review
- Ectopically expressed leaf and bulb lectins from garlic (Allium sativum L.) protect transgenic tobacco plants against cotton leafworm (Spodoptera littoralis).Transgenic research · 2008Article
- Arabidopsis vegetative storage protein is an anti-insect acid phosphatase.Plant physiology · 2005Article
- Plant lectins: occurrence, biochemistry, functions and applications.Glycoconjugate journal · 2001Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Griffonia simplicifolia leaf lectin II (GSII), a plant defense protein against certain insects, consists of an N-acetylglucosamine (GlcNAc)-binding large subunit with a small subunit having sequence homology to class III chitinases. Much of the insecticidal activity of GSII is attributable to the large lectin subunit, because bacterially expressed recombinant large subunit (rGSII) inhibited growth and development of the cowpea bruchid, Callosobruchus maculatus (F). Site-specific mutations were introduced into rGSII to generate proteins with altered GlcNAc binding, and the different rGSII proteins were evaluated for insecticidal activity when added to the diet of the cowpea bruchid. At pH 5.5, close to the physiological pH of the cowpea bruchid midgut lumen, rGSII recombinant proteins were categorized as having high (rGSII, rGSII-Y134F, and rGSII-N196D mutant proteins), low (rGSII-N136D), or no (rGSII-D88N, rGSII-Y134G, rGSII-Y134D, and rGSII-N136Q) GlcNAc-binding activity. Insecticidal activity of the recombinant proteins correlated with their GlcNAc-binding activity. Furthermore, insecticidal activity correlated with the resistance to proteolytic degradation by cowpea bruchid midgut extracts and with GlcNAc-specific binding to the insect digestive tract. Together, these results establish that insecticidal activity of GSII is functionally linked to carbohydrate binding, presumably to the midgut epithelium or the peritrophic matrix, and to biochemical stability of the protein to digestive proteolysis.
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