ArticleProceedings of the National Academy of Sciences of the United States of America1998
Mobility of cytochrome P450 in the endoplasmic reticulum membrane.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 62 citations in OpenAlex.
- E-Syt1 recruits PI4KA to endoplasmic reticulum-plasma membrane junctions to enhance PI4P synthesis.Science China. Life sciences · 2026Article
- Genome-wide analysis of theFrontiers in plant science · 2024Article
- Discovery of a monomeric green fluorescent protein sensor for chloride by structure-guided bioinformatics.Chemical science · 2022Article
- Massively parallel characterization of CYP2C9 variant enzyme activity and abundance.American journal of human genetics · 2021Article
- The functional effects of physical interactions involving cytochromes P450: putative mechanisms of action and the extent of these effects in biological membranes.Drug metabolism reviews · 2016Review
- Article
- Brassinosteroids regulate plant growth through distinct signaling pathways in Selaginella and Arabidopsis.PloS one · 2013Article
- Protein-protein and protein-membrane associations in the lignin pathway.The Plant cell · 2012Article
- Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions.Traffic (Copenhagen, Denmark) · 2012Article
- Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The signal-anchor sequence of CYP2C1 inserts into the membrane as a hairpin structure.Biochemical and biophysical research communications · 2011Article
- Protein kinase A activity at the endoplasmic reticulum surface is responsible for augmentation of human ether-a-go-go-related gene product (HERG).The Journal of biological chemistry · 2011Article
- Progesterone receptor membrane component 1 inhibits the activity of drug-metabolizing cytochromes P450 and binds to cytochrome P450 reductase.Molecular pharmacology · 2011Article
- Proteasome inhibition compromises direct retention of cytochrome P450 2C2 in the endoplasmic reticulum.Experimental cell research · 2008Article
- Agonist-evoked inositol trisphosphate receptor (IP3R) clustering is not dependent on changes in the structure of the endoplasmic reticulum.The Biochemical journal · 2006Article
- Derivation of a closed form analytical expression for fluorescence recovery after photo bleaching in the case of continuous bleaching during read out.The European physical journal. E, Soft matter · 2005Article
- Formation of stacked ER cisternae by low affinity protein interactions.The Journal of cell biology · 2003Article
- Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment.The Journal of cell biology · 2001Article
- Diffusion in inhomogeneous media: theory and simulations applied to whole cell photobleach recovery.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cytochrome P450 2C2 is a resident endoplasmic reticulum (ER) membrane protein that is excluded from the recycling pathway and contains redundant retention functions in its N-terminal transmembrane signal/anchor sequence and its large, cytoplasmic domain. Unlike some ER resident proteins, cytochrome P450 2C2 does not contain any known retention/retrieval signals. One hypothesis to explain exclusion of resident ER proteins from the transport pathway is the formation of networks by interaction with other proteins that immobilize the proteins and are incompatible with packaging into the transport vesicles. To determine the mobility of cytochrome P450 in the ER membrane, chimeric proteins of either cytochrome P450 2C2, its catalytic domain, or the cytochrome P450 2C1 N-terminal signal/anchor sequence fused to green fluorescent protein (GFP) were expressed in transiently transfected COS1 cells. The laurate hydroxylase activities of cytochrome P450 2C2 or the catalytic domain with GFP fused to the C terminus were similar to the native enzyme. The mobilities of the proteins in the membrane were determined by recovery of fluorescence after photobleaching. Diffusion coefficients for all P450 chimeras were similar, ranging from 2.6 to 6.2 x 10(-10) cm2/s. A coefficient only slightly larger (7.1 x 10(-10) cm2/s) was determined for a GFP chimera that contained a C-terminal dilysine ER retention signal and entered the recycling pathway. These data indicate that exclusion of cytochrome P450 from the recycling pathway is not mediated by immobilization in large protein complexes.
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