ArticleProceedings of the National Academy of Sciences of the United States of America1998
Attachment to the nuclear matrix mediates specific alterations in chromatin structure.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- DNase I Chromatin Accessibility Analysis.Bio-protocol · 2019Article
- Orc5 induces large-scale chromatin decondensation in a GCN5-dependent manner.Journal of cell science · 2016Article
- Chromatin unfolding by Cdt1 regulates MCM loading via opposing functions of HBO1 and HDAC11-geminin.Cell cycle (Georgetown, Tex.) · 2010Article
- Temporal differences in DNA replication during the S phase using single fiber analysis of normal human fibroblasts and glioblastoma T98G cells.Cell cycle (Georgetown, Tex.) · 2009Article
- The human stress-activated protein kin17 belongs to the multiprotein DNA replication complex and associates in vivo with mammalian replication origins.Molecular and cellular biology · 2005Article
- Genomic imprinting controls matrix attachment regions in the Igf2 gene.Molecular and cellular biology · 2003Article
- Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration.Nucleic acids research · 2003Article
- The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies.Molecular and cellular biology · 2002Article
- Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle.Nucleic acids research · 2001Article
- The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.Molecular and cellular biology · 2001Article
- Nuclear organization of DNA replication in primary mammalian cells.Genes & development · 2000Article
- Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies.The EMBO journal · 2000Article
- An origin of bidirectional DNA replication is located within a CpG island at the 3" end of the chicken lysozyme gene.Nucleic acids research · 1999Article
- [Regulation of DNA replication timing].Molekuliarnaia biologiiaReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The DNA in eukaryotic chromosomes is organized into a series of loops that are permanently attached at their bases to the nuclear scaffold or matrix at sequences known as scaffold-attachment or matrix-attachment regions. At present, it is not clear what effect affixation to the nuclear matrix has on chromatin architecture in important regulatory regions such as origins of replication or the promoter regions of genes. In the present study, we have investigated cell-cycle-dependent changes in the chromatin structure of a well characterized replication initiation zone in the amplified dihydrofolate reductase domain of the methotrexate-resistant Chinese hamster ovary cell line CHOC 400. Replication can initiate at any of multiple potential sites scattered throughout the 55-kilobase intergenic region in this domain, with two subregions (termed ori-beta and ori-gamma) being somewhat preferred. We show here that the chromatin in the ori-beta and ori-gamma regions undergoes dramatic alterations in micrococcal nuclease hypersensitivity as cells cross the G1/S boundary, but only in those copies of the amplicon that are affixed to the nuclear matrix. In contrast, the fine structure of chromatin in the promoter of the dihydrofolate reductase gene does not change detectably as a function of matrix attachment or cell-cycle position. We suggest that attachment of DNA to the nuclear matrix plays an important role in modulating chromatin architecture, and this could facilitate the activity of origins of replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.