ArticleThe Plant cell1998
Cell cycle -dependent proteolysis in plants. Identification Of the destruction box pathway and metaphase arrest produced by the proteasome inhibitor mg132
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 211 citations in OpenAlex.
- Stomatal XVE: an inducible system for cell-stage-specific gene expression and editing in the stomatal lineage.The New phytologist · 2026Article
- Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity.The Plant cell · 2025Article
- Sucrose preferentially promotes expression ofFrontiers in plant science · 2023Article
- Control of division in Chlamydomonas by cyclin B/CDKB1 and the anaphase-promoting complex.PLoS genetics · 2022Article
- The Arabidopsis stomatal polarity protein BASL mediates distinct processes before and after cell division to coordinate cell size and fate asymmetries.Development (Cambridge, England) · 2021Article
- Article
- The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development.Frontiers in plant science · 2021Article
- Editor Profile: Pascal Genschik.The Plant cell · 2020Article
- Proteasome inhibition rapidly exacerbates photoinhibition and impedes recovery during high light stress in Chlamydomonas reinhardtii.BMC plant biology · 2020Article
- Expression of Arabidopsis WEE1 in tobacco induces unexpected morphological and developmental changes.Scientific reports · 2019Article
- Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.Nature plants · 2019Article
- The RING-Type E3 Ligase XBAT35.2 Is Involved in Cell Death Induction and Pathogen Response.Plant physiology · 2017Article
- Identification and characterization of an efficient acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) gene from the microalga Chlorella ellipsoidea.BMC plant biology · 2017Article
- Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells.Plant molecular biology · 2016Article
- Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme.BMC plant biology · 2015Article
- Characterization of a null allelic mutant of the rice NAL1 gene reveals its role in regulating cell division.PloS one · 2015Article
- Composition, roles, and regulation of cullin-based ubiquitin e3 ligases.The arabidopsis book · 2014Article
- Plant WEE1 kinase is cell cycle regulated and removed at mitosis via the 26S proteasome machinery.Journal of experimental botany · 2013Article
- S phase of the cell cycle: a key phase for the regulation of thermodormancy in barley grain.Journal of experimental botany · 2012Article
- Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is widely assumed that mitotic cyclins are rapidly degraded during anaphase, leading to the inactivation of the cell cycle-dependent protein kinase Cdc2 and allowing exit from mitosis. The proteolysis of mitotic cyclins is ubiquitin/26S proteasome mediated and requires the presence of the destruction box motif at the N terminus of the proteins. As a first attempt to study cyclin proteolysis during the plant cell cycle, we investigated the stability of fusion proteins in which the N-terminal domains of an A-type and a B-type tobacco mitotic cyclin were fused in frame with the chloramphenicol acetyltransferase (CAT ) reporter gene and constitutively expressed in transformed tobacco BY2 cells. For both cyclin types, the N-terminal domains led the chimeric cyclin-CAT fusion proteins to oscillate in a cell cycle-specific manner. Mutations within the destruction box abolished cell cycle-specific proteolysis. Although both fusion proteins were degraded after metaphase, cyclin A-CAT proteolysis was turned off during S phase, whereas that of cyclin B-CAT was turned off only during the late G2 phase. Thus, we demonstrated that mitotic cyclins in plants are subjected to post-translational control (e.g., proteolysis). Moreover, we showed that the proteasome inhibitor MG132 blocks BY2 cells during metaphase in a reversible way. During this mitotic arrest, both cyclin-CAT fusion proteins remained stable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.