ArticleApplied and environmental microbiology1998
Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 65 citations in OpenAlex.
- Screening for probiotic characters in lactobacilli isolated from chickens revealed the intra-species diversity ofAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Molecular Routes to Specific Identification of the Lactobacillus Casei Group at the Species, Subspecies and Strain Level.International journal of molecular sciences · 2020Article
- Molecular Detection of Two Potential Probiotic Lactobacilli Strains and Evaluation of Their Performance as Starter Adjuncts in Yogurt Production.International journal of molecular sciences · 2016Article
- Detection and Identification of Probiotic Lactobacillus plantarum Strains by Multiplex PCR Using RAPD-Derived Primers.International journal of molecular sciences · 2015Article
- Highly Heterogeneous Probiotic Lactobacillus Species in Healthy Iranians with Low Functional Activities.PloS one · 2015Article
- Article
- Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.Applied and environmental microbiology · 2011Article
- Comparison of Phenotypic and Genotypic Methods Used for the Species Identification of Lactobacillus NP51 and Development of a Strain-Specific PCR Assay.Probiotics and antimicrobial proteins · 2010Article
- Development of a sequence-characterized amplified region marker-targeted quantitative PCR assay for strain-specific detection of Oenococcus oeni during wine malolactic fermentation.Applied and environmental microbiology · 2010Article
- Are probiotics detectable in human feces after oral uptake by healthy volunteers?Wiener klinische Wochenschrift · 2007Article
- Characterization of six Leuconostoc fallax bacteriophages isolated from an industrial sauerkraut fermentation.Applied and environmental microbiology · 2002Article
- Persistence of Lactobacillus fermentum RC-14 and Lactobacillus rhamnosus GR-1 but not L. rhamnosus GG in the human vagina as demonstrated by randomly amplified polymorphic DNA.Clinical and diagnostic laboratory immunology · 2002Article
- Randomly amplified polymorphic DNA analysis of Erysipelothrix spp.Journal of clinical microbiology · 2000Article
- PCR assays that identify the grapevine dieback fungus Eutypa lata.Applied and environmental microbiology · 2000Article
- Specific detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA primers identified by randomly amplified polymorphic DNA analysis.Applied and environmental microbiology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L. rhamnosus Lc 1/3 was constructed based on the sequence. The primer pair was tested with 11 Lactobacillus species and 11 L. rhamnosus strains and was found to be strain specific. The nucleotide sequence of the specific RAPD marker was found to contain part of a protein encoding region which showed significant similarity to several transposases for insertion sequence elements of various bacteria, including other lactic acid bacterium species.
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