ArticleThe Journal of general physiology1998
Voltage-dependent gating of single wild-type and S4 mutant KAT1 inward rectifier potassium channels.
Article in The Journal of general physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Research Progress on Plant Shaker KPlants (Basel, Switzerland) · 2024Review
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- External Cd2+ and protons activate the hyperpolarization-gated K+ channel KAT1 at the voltage sensor.The Journal of general physiology · 2021Article
- Gating control and KPlant, cell & environment · 2018Article
- Fusicoccin Activates KAT1 Channels by Stabilizing Their Interaction with 14-3-3 Proteins.The Plant cell · 2017Article
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor.Plant physiology · 2014Article
- Effects of strontium on the permeation and gating phenotype of calcium channels in hair cells.Journal of neurophysiology · 2008Article
- A unique voltage sensor sensitizes the potassium channel AKT2 to phosphoregulation.The Journal of general physiology · 2005Article
- Fast, triangular voltage clamp for recording and kinetic analysis of an ion transporter expressed in Xenopus oocytes.Biophysical journal · 2005Article
- Orientation of Arabidopsis thaliana KAT1 channel in the plasma membrane.The Journal of membrane biology · 2004Article
- Voltage-dependent gating of the cystic fibrosis transmembrane conductance regulator Cl- channel.The Journal of general physiology · 2003Article
- Molecular coupling between voltage sensor and pore opening in the Arabidopsis inward rectifier K+ channel KAT1.The Journal of general physiology · 2003Article
- Effects of permeant ion concentrations on the gating of L-type Ca2+ channels in hair cells.Biophysical journal · 2003Article
- Regulated expression of Arabidopsis shaker K+ channel genes involved in K+ uptake and distribution in the plant.Plant molecular biology · 2003Article
- The search is on for the voltage sensor-to-gate coupling.The Journal of general physiology · 2002Review
- A grapevine gene encoding a guard cell K(+) channel displays developmental regulation in the grapevine berry.Plant physiology · 2002Article
- Extracellular protons inhibit the activity of inward-rectifying potassium channels in the motor cells of Samanea saman pulvini.Plant physiology · 2001Article
- The S4-S5 linker couples voltage sensing and activation of pacemaker channels.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Direct measurement of single-channel Ca(2+) currents in bullfrog hair cells reveals two distinct channel subtypes.The Journal of physiology · 2001Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The voltage-dependent gating mechanism of KAT1 inward rectifier potassium channels was studied using single channel current recordings from Xenopus oocytes injected with KAT1 mRNA. The inward rectification properties of KAT1 result from an intrinsic gating mechanism in the KAT1 channel protein, not from pore block by an extrinsic cation species. KAT1 channels activate with hyperpolarizing potentials from -110 through -190 mV with a slow voltage-dependent time course. Transitions before first opening are voltage dependent and account for much of the voltage dependence of activation, while transitions after first opening are only slightly voltage dependent. Using burst analysis, transitions near the open state were analyzed in detail. A kinetic model with multiple closed states before first opening, a single open state, a single closed state after first opening, and a closed-state inactivation pathway accurately describes the single channel and macroscopic data. Two mutations neutralizing charged residues in the S4 region (R177Q and R176L) were introduced, and their effects on single channel gating properties were examined. Both mutations resulted in depolarizing shifts in the steady state conductance-voltage relationship, shortened first latencies to opening, decreased probability of terminating bursts, and increased burst durations. These effects on gating were well described by changes in the rate constants in the kinetic model describing KAT1 channel gating. All transitions before the open state were affected by the mutations, while the transitions after the open state were unaffected, implying that the S4 region contributes to the early steps in gating for KAT1 channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.