ArticleGenetics1998
The anatomy of a hypoxic operator in Saccharomyces cerevisiae.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Replication-IDentifier links epigenetic and metabolic pathways to the replication stress response.Nature communications · 2025Article
- Defects in mitochondrial fatty acid synthesis result in failure of multiple aspects of mitochondrial biogenesis in Saccharomyces cerevisiae.Molecular microbiology · 2013Article
- Gene responses to oxygen availability in Kluyveromyces lactis: an insight on the evolution of the oxygen-responding system in yeast.PloS one · 2009Article
- Frequent gain and loss of functional transcription factor binding sites.PLoS computational biology · 2007Article
- Metabolic-state-dependent remodeling of the transcriptome in response to anoxia and subsequent reoxygenation in Saccharomyces cerevisiae.Eukaryotic cell · 2006Article
- Synergy among differentially regulated repressors of the ribonucleotide diphosphate reductase genes of Saccharomyces cerevisiae.Eukaryotic cell · 2006Article
- Dynamical remodeling of the transcriptome during short-term anaerobiosis in Saccharomyces cerevisiae: differential response and role of Msn2 and/or Msn4 and other factors in galactose and glucose media.Molecular and cellular biology · 2005Article
- Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3.Eukaryotic cell · 2005Article
- Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
- Enriching for direct regulatory targets in perturbed gene-expression profiles.Genome biology · 2004Article
- Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein.Eukaryotic cell · 2003Article
- Genomic analyses of anaerobically induced genes in Saccharomyces cerevisiae: functional roles of Rox1 and other factors in mediating the anoxic response.Journal of bacteriology · 2002Article
- Article
- Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Characterization of Rny1, the Saccharomyces cerevisiae member of the T2 RNase family of RNases: unexpected functions for ancient enzymes?Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- DNA binding by single HMG box model proteins.Nucleic acids research · 2000Article
- Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast.Molecular and cellular biology · 2000Article
- Building a dictionary for genomes: identification of presumptive regulatory sites by statistical analysis.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast.The EMBO journal · 2000Article
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5 authors.
Funding
Abstract
Aerobic repression of the hypoxic genes of Saccharomyces cerevisiae is mediated by the DNA-binding protein Rox1 and the Tup1/Ssn6 general repression complex. To determine the DNA sequence requirements for repression, we carried out a mutational analysis of the consensus Rox1-binding site and an analysis of the arrangement of the Rox1 sites into operators in the hypoxic ANB1 gene. We found that single base pair substitutions in the consensus sequence resulted in lower affinities for Rox1, and the decreased affinity of Rox1 for mutant sites correlated with the ability of these sites to repress expression of the hypoxic ANB1 gene. In addition, there was a general but not complete correlation between the strength of repression of a given hypoxic gene and the compliance of the Rox1 sites in that gene to the consensus sequence. An analysis of the ANB1 operators revealed that the two Rox1 sites within an operator acted synergistically in vivo, but that Rox1 did not bind cooperatively in vitro, suggesting the presence of a higher order repression complex in the cell. In addition, the spacing or helical phasing of the Rox1 sites was not important in repression. The differential repression by the two operators of the ANB1 gene was found to be due partly to the location of the operators and partly to the sequences between the two Rox1-binding sites in each. Finally, while Rox1 repression requires the Tup1/Ssn6 general repression complex and this complex has been proposed to require the aminoterminal regions of histones H3 and H4 for full repression of a number of genes, we found that these regions were dispensable for ANB1 repression and the repression of two other hypoxic genes.
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