ArticleJournal of bacteriology1998
The Bacillus subtilis nucleotidyltransferase is a tRNA CCA-adding enzyme.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- Distribution of poly(A) polymerase I in bacteria: an expanded role for horizontal gene transfer.Microbiology (Reading, England) · 2026Article
- Acquisition ofMicrobial genomics · 2021Article
- Analysis of tRNAF1000Research · 2020Article
- Phylogeny and Evolution of RNA 3'-Nucleotidyltransferases in Bacteria.Journal of molecular evolution · 2019Article
- New Insights into the Relationship between tRNA Processing and Polyadenylation in Escherichia coli.Trends in genetics : TIG · 2019Review
- Critical Minireview: The Fate of tRNABiomolecules · 2017Review
- Unusual evolution of a catalytic core element in CCA-adding enzymes.Nucleic acids research · 2010Article
- tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.Cellular and molecular life sciences : CMLS · 2010Review
- A comparative analysis of two conserved motifs in bacterial poly(A) polymerase and CCA-adding enzyme.Nucleic acids research · 2008Article
- Evolution of tRNA nucleotidyltransferases: a small deletion generated CC-adding enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Polyadenylation of a functional mRNA controls gene expression in Escherichia coli.Nucleic acids research · 2007Article
- A phylogeny of bacterial RNA nucleotidyltransferases: Bacillus halodurans contains two tRNA nucleotidyltransferases.Journal of bacteriology · 2005Article
- Addition of poly(A) and heteropolymeric 3' ends in Bacillus subtilis wild-type and polynucleotide phosphorylase-deficient strains.Journal of bacteriology · 2005Article
- Sequence motifs that distinguish ATP(CTP):tRNA nucleotidyl transferases from eubacterial poly(A) polymerases.RNA (New York, N.Y.) · 2004Article
- Mitochondrial tRNA 3' end metabolism and human disease.Nucleic acids research · 2004Review
- The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA.Journal of bacteriology · 2003Article
- RNA processing and degradation in Bacillus subtilis.Microbiology and molecular biology reviews : MMBR · 2003Review
- Polynucleotide phosphorylase functions as both an exonuclease and a poly(A) polymerase in spinach chloroplasts.Molecular and cellular biology · 2001Article
- Addition of non-genomically encoded nucleotides to the 3'-terminus of maize mitochondrial mRNAs: truncated rps12 mRNAs frequently terminate with CCA.Nucleic acids research · 2000Article
- Characterization of the E.coli poly(A) polymerase: nucleotide specificity, RNA-binding affinities and RNA structure dependence.Nucleic acids research · 2000Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There has been increased interest in bacterial polyadenylation with the recent demonstration that 3' poly(A) tails are involved in RNA degradation. Poly(A) polymerase I (PAP I) of Escherichia coli is a member of the nucleotidyltransferase (Ntr) family that includes the functionally related tRNA CCA-adding enzymes. Thirty members of the Ntr family were detected in a search of the current database of eubacterial genomic sequences. Gram-negative organisms from the beta and gamma subdivisions of the purple bacteria have two genes encoding putative Ntr proteins, and it was possible to predict their activities as either PAP or CCA adding by sequence comparisons with the E. coli homologues. Prediction of the functions of proteins encoded by the genes from more distantly related bacteria was not reliable. The Bacillus subtilis papS gene encodes a protein that was predicted to have PAP activity. We have overexpressed and characterized this protein, demonstrating that it is a tRNA nucleotidyltransferase. We suggest that the papS gene should be renamed cca, following the notation for its E. coli counterpart. The available evidence indicates that cca is the only gene encoding an Ntr protein, despite previous suggestions that B. subtilis has a PAP similar to E. coli PAP I. Thus, the activity involved in RNA 3' polyadenylation in the gram-positive bacteria apparently resides in an enzyme distinct from its counterpart in gram-negative bacteria.
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