ArticleProceedings of the National Academy of Sciences of the United States of America1998
Stoichiometry and arrangement of heteromeric olfactory cyclic nucleotide-gated ion channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Reconstructing calcium conductance in MCUb illuminates its molecular design for tuning the mitochondrial calcium uniporter.Nature communications · 2026Article
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- Determining the correct stoichiometry of Kv2.1/Kv6.4 heterotetramers, functional in multiple stoichiometrical configurations.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Functional Analysis of Orai1 Concatemers Supports a Hexameric Stoichiometry for the CRAC Channel.Biophysical journal · 2016Article
- Stoichiometry of the human glycine receptor revealed by direct subunit counting.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Chemical structures of odorants that suppress ion channels in the olfactory receptor cell.The journal of physiological sciences : JPS · 2011Article
- How to validate a heteromeric ion channel drug target: assessing proper expression of concatenated subunits.The Journal of general physiology · 2008Article
- Article
- Efficient coupling of ligand binding to channel opening by the binding domain of a modulatory (beta) subunit of the olfactory cyclic nucleotide-gated channel.The Journal of general physiology · 2001Article
- Molecular regions controlling the activity of CNG channels.The Journal of general physiology · 2001Article
- Interactions of cyclic nucleotide-gated channel subunits and protein tyrosine kinase probed with genistein.The Journal of general physiology · 2000Article
- Structural basis for ligand selectivity of heteromeric olfactory cyclic nucleotide-gated channels.Biophysical journal · 2000Article
- Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Tuning Ca2+ permeation in cyclic nucleotide-gated channels.The Journal of general physiology · 1999Review
- Divalent cation selectivity is a function of gating in native and recombinant cyclic nucleotide-gated ion channels from retinal photoreceptors.The Journal of general physiology · 1999Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Native cylic nucleotide-gated (CNG) channels are composed of alpha and beta subunits. Olfactory CNG channels were expressed from rat cDNA clones in Xenopus oocytes and studied in inside-out patches. Using tandem dimers composed of linked subunits, we investigated the stoichiometry and arrangement of the alpha and beta subunits. Dimers contained three subunit types: alphawt, betawt, and alpham. The alpham subunit lacks an amino-terminal domain that greatly influences gating, decreasing the apparent affinity of the channel for ligand by 9-fold, making it a reporter for inclusion in the tetramer. Homomeric channels from injection of alphawtalphawt dimers and from alphawt monomers were indistinguishable. Channels from injection of alphawtalpham dimers had apparent affinities 3-fold lower than alphawt homomultimers, suggesting a channel with two alphawt and two alpham subunits. Channels from coinjection of alphawtalphawt and betabeta dimers were indistinguishable from those composed of alpha and beta monomers and shared all of the characteristics of the alpha+beta phenotype of heteromeric channels. Coinjection of alphawtalpham and beta beta dimers yielded channels also of the alpha+beta phenotype but with an apparent affinity 3-fold lower, indicating the presence of alpham in the tetramer and that alpha+beta channels have adjacent alpha-subunits. To distinguish between an alpha-alpha-alpha-beta and an alpha-alpha-beta-beta arrangement, we compared apparent affinities for channels from coinjection of alphawtalphawt and betaalphawt or alphawtalphawt and betaalpham dimers. These channels were indistinguishable. To further argue against an alpha-alpha-alpha-beta arrangement, we quantitatively compared dose-response data for channels from coinjection of alphawtalpham and beta beta dimers to those from alpha and beta monomers. Taken together, our results are most consistent with an alpha-alpha-beta-beta arrangement for the heteromeric olfactory CNG channel.
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