ArticleProceedings of the National Academy of Sciences of the United States of America1998
Autoregulation at the level of mRNA 3' end formation of the suppressor of forked gene of Drosophila melanogaster is conserved in Drosophila virilis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Regulation of gene expression by alternative polyadenylation in health and disease.Nature reviews. Genetics · 2026Review
- Identification and Functional Analysis of ThreeInsects · 2024Article
- Insights into Peptidyl-ProlylPathogens (Basel, Switzerland) · 2024Review
- 3'-End Processing of Eukaryotic mRNA: Machinery, Regulation, and Impact on Gene Expression.Annual review of biochemistry · 2023Review
- The Green Valley ofCells · 2022Review
- Transcriptional Control by Premature Termination: A Forgotten Mechanism.Trends in genetics : TIG · 2019Review
- Molecular Regulation of Alternative Polyadenylation (APA) within the Drosophila Nervous System.Journal of molecular biology · 2017Article
- Alternative polyadenylation of mRNA precursors.Nature reviews. Molecular cell biology · 2017Review
- The conserved intronic cleavage and polyadenylation site of CstF-77 gene imparts control of 3' end processing activity through feedback autoregulation and by U1 snRNP.PLoS genetics · 2013Article
- PABPN1 shuts down alternative poly(A) sites.Cell research · 2012Article
- RNA polymerase II kinetics in polo polyadenylation signal selection.The EMBO journal · 2011Article
- Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.Nucleic acids research · 2007Article
- Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3'-UTRs.Nucleic acids research · 2006Article
- A nuclear surveillance pathway for mRNAs with defective polyadenylation.Molecular and cellular biology · 2005Article
- Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Probabilistic prediction of Saccharomyces cerevisiae mRNA 3'-processing sites.Nucleic acids research · 2002Article
- Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation.Molecular and cellular biology · 2001Article
- Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Tissue-specific autoregulation of Drosophila suppressor of forked by alternative poly(A) site utilization leads to accumulation of the suppressor of forked protein in mitotically active cells.RNA (New York, N.Y.) · 2000Article
- In silico detection of control signals: mRNA 3'-end-processing sequences in diverse species.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila melanogaster Suppressor of forked [Su(f)] protein shares homology with the yeast RNA14 protein and the 77-kDa subunit of human cleavage stimulation factor, which are proteins involved in mRNA 3' end formation. This suggests a role for Su(f) in mRNA 3' end formation in Drosophila. The su(f) gene produces three transcripts; two of them are polyadenylated at the end of the transcription unit, and one is a truncated transcript, polyadenylated in intron 4. Using temperature-sensitive su(f) mutants, we show that accumulation of the truncated transcript requires wild-type Su(f) protein. This suggests that the Su(f) protein autoregulates negatively its accumulation by stimulating 3' end formation of the truncated su(f) RNA. Cloning of su(f) from Drosophila virilis and analysis of its RNA profile suggest that su(f) autoregulation is conserved in this species. Sequence comparison between su(f) from both species allows us to point out three conserved regions in intron 4 downstream of the truncated RNA poly(A) site. These conserved regions include the GU-rich downstream sequence involved in poly(A) site definition. Using transgenes truncated within intron 4, we show that sequence up to the conserved GU-rich domain is sufficient for production of the truncated RNA and for regulation of this production by su(f). Our results indicate a role of su(f) in the regulation of poly(A) site utilization and an important role of the GU-rich sequence for this regulation to occur.
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