ArticleProceedings of the National Academy of Sciences of the United States of America1998
An artificial cell-cycle inhibitor isolated from a combinatorial library.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 111 citations in OpenAlex.
- M1-NP1 interfering-peptide inhibits cancer cell proliferation and migration by targeting the transcription factor FOXM1.Journal of pharmaceutical analysis · 2026Article
- Aptamer-based Emerging Tools for Viral Biomarker Detection: A Focus on SARS-CoV-2.Current medicinal chemistry · 2023Review
- Interaction of Peptide Aptamers with Prion Protein Central Domain Promotes α-Cleavage of PrPMolecular neurobiology · 2018Article
- A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin.Sensors (Basel, Switzerland) · 2016Article
- MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing.Biosensors · 2016Review
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
- Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics.Molecular biology of the cell · 2014Article
- Aptamers and their potential to selectively target aspects of EGF, Wnt/β-catenin and TGFβ-smad family signaling.International journal of molecular sciences · 2013Review
- Combinatorial library of improved peptide aptamers, CLIPs to inhibit RAGE signal transduction in mammalian cells.PloS one · 2013Article
- Article
- Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation.BMC cell biology · 2010Article
- Structure-function studies of an engineered scaffold protein derived from stefin A. I: Development of the SQM variant.Protein engineering, design & selection : PEDS · 2010Article
- Analysis of intracellular substrates and products of thimet oligopeptidase in human embryonic kidney 293 cells.The Journal of biological chemistry · 2009Article
- Electrical protein detection in cell lysates using high-density peptide-aptamer microarrays.Journal of biology · 2008Article
- The eleven-year switch of peptide aptamers.Journal of biology · 2008Review
- Modulation of Nr-13 antideath activity by peptide aptamers.Oncogene · 2007Article
- Ligand-regulated peptide aptamers that inhibit the 5'-AMP-activated protein kinase.Journal of molecular biology · 2007Article
- Inhibition of transforming growth factor-beta1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer Trx-SARA.Molecular biology of the cell · 2006Article
- Peptide aptamers that bind to a geminivirus replication protein interfere with viral replication in plant cells.Journal of virology · 2006Article
- Bifunctional recombinant proteins in cancer therapy: cell penetrating peptide aptamers as inhibitors of growth factor signaling.Journal of cancer research and clinical oncology · 2003Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the genetic networks that operate inside cells will require the dissection of interactions among network members. Here we describe a peptide aptamer isolated from a combinatorial library that distinguishes among such interactions. This aptamer binds to cyclin-dependent kinase 2 (Cdk2) and inhibits its kinase activity. In contrast to naturally occurring inhibitors, such as p21(Cip1), which inhibit the activity of Cdk2 on all its substrates, inhibition by pep8 has distinct substrate specificity. We show that the aptamer binds to Cdk2 at or near its active site and that its mode of inhibition is competitive. Expression of pep8 in human cells retards their progression through the G1 phase of the cell cycle. Our results suggest that the aptamer inhibits cell-cycle progression by blocking the activity of Cdk2 on substrates needed for the G1-to-S transition. This work demonstrates the feasibility of selection of artificial proteins to perform functions not developed during evolution. The ability to select proteins that block interactions between a gene product and some partners but not others should make sophisticated genetic manipulations possible in human cells and other currently intractable systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.