ArticleInfection and immunity1998
Analysis of clinical and environmental strains of nontoxigenic Vibrio cholerae for susceptibility to CTXPhi: molecular basis for origination of new strains with epidemic potential.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 104 citations in OpenAlex.
- Genomic characterization ofFrontiers in microbiology · 2025Article
- Quorum regulated latent environmental cells of toxigenic Vibrio cholerae and their role in cholera outbreaks.Gut pathogens · 2024Review
- Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance.Archives of microbiology · 2022Review
- Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains.Microbiology resource announcements · 2018Article
- Electrostatic interactions between the CTX phage minor coat protein and the bacterial host receptor TolA drive the pathogenic conversion ofThe Journal of biological chemistry · 2017Article
- Retrospective genomic analysis of Vibrio cholerae O1 El Tor strains from different places in India reveals the presence of ctxB-7 allele found in Haitian isolates.Epidemiology and infection · 2017Article
- Replication ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Hybrid Pre-CTXΦ-RS1 Prophage Genome and Its Regulatory Function in Environmental Vibrio cholerae O1 Strains.Applied and environmental microbiology · 2015Article
- RS1 satellite phage promotes diversity of toxigenic Vibrio cholerae by driving CTX prophage loss and elimination of lysogenic immunity.Infection and immunity · 2014Article
- The type II secretion pathway in Vibrio cholerae is characterized by growth phase-dependent expression of exoprotein genes and is positively regulated by σE.Infection and immunity · 2014Article
- Proteomic analysis of Vibrio cholerae outer membrane vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Assessment of the relationship between bacteriological quality of dug-wells, hygiene behaviour and well characteristics in two cholera endemic localities in Douala, Cameroon.BMC public health · 2013Article
- Review
- Characterization of novel alleles of toxin co-regulated pilus A gene (tcpA) from environmental isolates of Vibrio cholerae.Current microbiology · 2011Article
- Cholera and climate: a demonstrated relationship.Transactions of the American Clinical and Climatological Association · 2009Article
- The coastal environment and human health: microbial indicators, pathogens, sentinels and reservoirs.Environmental health : a global access science source · 2008Review
- Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux.BMC microbiology · 2008Article
- Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Distribution of genes for virulence and ecological fitness among diverse Vibrio cholerae population in a cholera endemic area: tracking the evolution of pathogenic strains.DNA and cell biology · 2008Article
- Contamination of potable water distribution systems by multiantimicrobial-resistant enterohemorrhagic Escherichia coli.Environmental health perspectives · 2008Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxigenic Vibrio cholerae strains are lysogens of CTXPhi, a filamentous phage which encodes cholera toxin. The receptor for CTXPhi for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 146 CTX-negative strains of V. cholerae O1 or non-O1 isolated from patients or surface waters in five different countries for the presence of the TCP pathogenicity island, the regulatory gene toxR, and the CTXPhi attachment sequence attRS, as well as for susceptibility of the strains to CTXPhi, to investigate the molecular basis for the emergence of new clones of toxigenic V. cholerae. DNA probe or PCR assays for tcpA, tcpI, acfB, toxR, and attRS revealed that 6.85% of the strains, all of which belonged to the O1 serogroup, carried the TCP pathogenicity island, toxR, and multiple copies of attRS, whereas the remaining 93.15% of the strains were negative for TCP but positive for either one or both or neither of toxR and attRS. An analysis of the strains for susceptibility to CTXPhi, using a genetically marked derivative of the phage CTX-KmPhi, showed that all TCP-positive CTX-negative strains and 1 of 136 TCP-negative strains were infected by the phage either in vitro or in the intestines of infant mice. The phage genome integrated into the chromosome of infected V. cholerae O1 cells forming stable lysogens. Comparative analysis of rRNA gene restriction patterns revealed that the lysogens derived from nontoxigenic progenitors were either closely related to or distinctly different from previously described clones of toxigenic V. cholerae. To our knowledge, this is the first demonstration of lysogenic conversion of naturally occurring nontoxigenic V. cholerae strains by CTXPhi. The results of this study further indicated that strains belonging to the O1 serogroup of V. cholerae are more likely to possess the TCP pathogenicity island and hence to be infected by CTXPhi, leading to the origination of potential new epidemic clones.
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