ArticleInfection and immunity1998
Cloning and sequencing of yajC and secD homologs of Brucella abortus and demonstration of immune responses to YajC in mice vaccinated with B. abortus RB51.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
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- Article
- The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.Journal of industrial microbiology & biotechnology · 2016Article
- Recent advances in Brucella abortus vaccines.Veterinary research · 2015Review
- Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide.Frontiers in cellular and infection microbiology · 2015Article
- Article
- Pluronic P85 enhances the efficacy of outer membrane vesicles as a subunit vaccine against Brucella melitensis challenge in mice.FEMS immunology and medical microbiology · 2012Article
- Article
- Characterization of recombinant B. abortus strain RB51SOD toward understanding the uncorrelated innate and adaptive immune responses induced by RB51SOD compared to its parent vaccine strain RB51.Frontiers in cellular and infection microbiology · 2011Article
- Identification of a single-nucleotide insertion in the promoter region affecting the sodC promoter activity in Brucella neotomae.PloS one · 2010Article
- Genome-wide profiling of humoral immune response to Coxiella burnetii infection by protein microarray.Proteomics · 2010Article
- Candidate antigens for Q fever serodiagnosis revealed by immunoscreening of a Coxiella burnetii protein microarray.Clinical and vaccine immunology : CVI · 2008Article
- Induction of antigen-specific Th1-type immune responses by gamma-irradiated recombinant Brucella abortus RB51.Clinical and diagnostic laboratory immunology · 2005Article
- Carboxyl-terminal protease regulates Brucella suis morphology in culture and persistence in macrophages and mice.Journal of bacteriology · 2005Article
- Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans.Clinical microbiology reviews · 2003Review
- Recombinant Ochrobactrum anthropi expressing Brucella abortus Cu,Zn superoxide dismutase protects mice against B. abortus infection only after switching of immune responses to Th1 type.Infection and immunity · 2002Article
- Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp.Infection and immunity · 2001Article
- Induction of specific cytotoxic lymphocytes in mice vaccinated with Brucella abortus RB51.Infection and immunity · 2001Article
- Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant.Infection and immunity · 2001Article
- Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in HeLa cells.Infection and immunity · 2001Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify Brucella antigens that are potentially involved in stimulating a protective cell-mediated immune response, a gene library of Brucella abortus 2308 was screened for the expression of antigens reacting with immunoglobulin G2a antibodies from BALB/c mice vaccinated with B. abortus RB51. One selected positive clone (clone MCB68) contained an insert of 2.6 kb; nucleotide sequence analysis of this insert revealed two open reading frames (ORFs). The deduced amino acid sequences of the first and second ORFs had significant similarities with the YajC and SecD proteins, respectively, of several bacterial species. Both the YajC and SecD proteins were expressed in Escherichia coli as fusion proteins with maltose binding protein (MBP). In Western blots, sera from mice vaccinated with B. abortus RB51 recognized YajC but not SecD. Further Western blot analysis with purified recombinant YajC protein indicated that mice inoculated with B. abortus 19 or 2308 or B. melitensis RM1 also produced antibodies to YajC. In response to in vitro stimulation with recombinant MBP-YajC fusion protein, splenocytes from mice vaccinated with B. abortus RB51 were able to proliferate and produce gamma interferon but not interleukin-4. This study demonstrates, for the first time, the involvement of YajC protein in an immune response to an infectious agent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.