ArticleThe Journal of physiology1998
Non-additive interaction between nicotinic cholinergic and P2X purine receptors in guinea-pig enteric neurons in culture.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 109 citations in OpenAlex.
- Copper Increases the Cooperative Gating of Rat P2X2a Receptor Channels.Pharmaceuticals (Basel, Switzerland) · 2024Article
- The gut brain in a dish: Murine primary enteric nervous system cell cultures.Neurogastroenterology and motility · 2022Review
- P2X receptors.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Subtype-specific mechanisms for functional interaction between α6β4* nicotinic acetylcholine receptors and P2X receptors.Molecular pharmacology · 2014Article
- Purinergic signalling in the gastrointestinal tract and related organs in health and disease.Purinergic signalling · 2014Review
- Review
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function.Journal of muscle research and cell motility · 2012Article
- Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Plasticity of non-adrenergic non-cholinergic bladder contractions in rats after chronic spinal cord injury.Brain research bulletin · 2011Article
- Regulation of GABA(A) receptor dynamics by interaction with purinergic P2X(2) receptors.The Journal of biological chemistry · 2011Article
- Patch clamp recording from enteric neurons in situ.Nature protocols · 2011Article
- GABA(A) Receptors: Post-Synaptic Co-Localization and Cross-Talk with Other Receptors.Frontiers in cellular neuroscience · 2011Article
- Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels.Neuron · 2010Article
- Molecular mechanisms of cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells.Neurogastroenterology and motility · 2010Article
- Cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Activation of cholinergic receptors blocks non-adrenergic non-cholinergic contractions in the rat urinary bladder.Brain research bulletin · 2008Article
- Purinergic receptors and synaptic transmission in enteric neurons.Purinergic signalling · 2008Article
- 5-HT4 receptor activation facilitates recovery from synaptic rundown and increases transmitter release from single varicosities of myenteric neurons.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- Interaction of P2 purinergic receptors with cellular macromolecules.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Acetylcholine (ACh)-activated currents and their interaction with ATP-activated currents were studied in primary cultures of myenteric neurons from guinea-pig small intestine using patch clamp techniques. Peak currents caused by co-application of ACh (1 mM) and ATP (300 microM) were 78 +/- 2 % of the sum of currents activated by each agonist alone (P < 0.05, n = 29). Reversal potentials measured during co-application of ACh and ATP did not differ from those measured during application of ACh or ATP alone. Addition of BAPTA (10 mM) to the pipette solution or replacement of extracellular Ca2+ with Na+ did not prevent occlusion. 2. Responses caused by co-application of 5-HT (300 microM), acting at 5-HT3 receptors, and ACh (3 mM) or ATP (1 mM) were additive (94 +/- 3 or 96 +/- 4 %, respectively, of the sum of currents activated by 5-HT and ACh or ATP alone; P > 0.05). Currents caused by GABA (1 mM), acting at GABAA receptors, and ACh (3 mM) or ATP (1 mM) were also additive (105 +/- 4 or 100 +/- 3 %, respectively, of the sum of currents activated by GABA and ACh or GABA and ATP applied separately; P > 0. 05). 3. Single channel currents caused by ACh and ATP in the same outside-out patches were less than additive (85 +/- 10 % of the predicted sum, P < 0.05). 4. P2X receptors and nicotinic cholinergic receptors (nAChRs) are linked in a mutually inhibitory manner in guinea-pig myenteric neurons. The functional interaction does not involve ligand binding sites, Ca2+-dependent mechanisms, a change in the driving force for Na+ or cytoplasmic signalling mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.